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Huntsman Research Breeding & Genetics Taxonomy Toxicology Animal Health Ocean Assets Visiting Scientist Program More Visiting Scientist Program The Huntsman Marine Science Centre has a globally recognized aquatic research program that focuses on Breeding & Genetics, Taxonomy & Biodiversity, Toxicology, and Animal Health . Our expertise and facilities have supported numerous collaborative multi-disciplinary research endeavors since our inception in 1969 with these efforts resulting in more than 500 contributed peer-reviewed publications over this time. The goals of this program are to: Help early career scientists to broaden their research perspective and develop/strengthen collaborative linkages within the Huntsman Marine core fields of research. Encourage senior scientists to share their lifelong research experiences while pursuing a specific passion project. Nurture long-term collaborative partnerships between Visiting Scientists and Huntsman Marine Research Scientists. Prioritize collaboration with researchers who are from underrepresented minorities in the ocean sciences and from Huntsman Marine Institutional Members through the application review and selection process. Program Overview Provided by Huntsman Marine Application Process Recommendations for Visiting Scientist Applicants Activities of and Benefits to Visiting Scientists Expectations from Visiting Scientists Evaluation Criteria Past Visiting Scientists Program Overview The Huntsman Marine Science Centre has a long history of hosting collaborators to complete ocean focused research. The Visiting Scientist Program was initiated in 2023 to provide a formal structure to welcome applications from academic, research centre and industry scientists at all levels of their professional development for short research visits and sabbaticals. Applicants focused on collaborative ocean-focused scientific activities that align with the research expertise of The Huntsman Marine Science Centre will be given priority through this program. Successful Visiting Scientists generally: Hold a doctoral degree or the equivalent with expertise that aligns with one of the four core disciplines of Huntsman Marine research (Note: last-year PhD candidates are also encouraged to apply but must be completing all requirements of their PhD program within one year of submitting their application); Presently hold or are between appointments comparable to that of a Huntsman Marine Research Scientist; and In most cases, during the tenure of the program, will be on temporary leave from their academic, research centre or industry position (such as a sabbatical or professional development leave) to ensure focus is directed towards your collaborative research while participating as a Huntsman Marine Visiting Scientist.

Land Development Huntsman owns about 11.5 hectares (28 acres) of prime land for development within its Upper Campus land holdings. The St. Andrews Town Council unanimously passed its Municipal Plan during a Special Meeting on 16-December-20, which designated this Huntsman property as an Institutional Growth Area to develop the Huntsman Ocean Park. This quantity of land provides a significant economic opportunity with up to 2,000 individuals employed by companies co-located within the Huntsman Ocean Park. St. Andrews, NB offers numerous assets that further increases the appeal to locate business activities within the Huntsman Ocean Park, including: Amazing community vibe with a variety of activities for the entire family encompassing nature & hiking, arts & culture, culinary, four season sports, and music & festival interests amongst so much more. Professional catering and event facilities offered by the Huntsman, including the Fundy Discovery Aquarium with its numerous Galleries to host spectacular events and state-of-the-art 218 seat Dunn Science Theatre within the most unique surroundings on the shores of the Bay of Fundy. Additional tourism focused attractions – such as Kingsbrae Gardens, Ministers Island and Sunbury Shores – with each promoting a series of events, festivals, concerts, and so much more throughout the year. Home of the Algonquin Resort – a 233 room Marriott Autograph Collection hotel – as well as numerous other accommodation options throughout our vibrant community that will facilitate year round business travel, symposia, and other business functions. The historic but recently redesigned award-winning 18-hole Algonquin Golf Course, with its clubhouse located within a short walking distance from the Huntsman Upper Campus property, to allow business visitors and those living here an opportunity to unwind. Presence of the fully-equipped St. Andrews New Brunswick Community College campus featuring a cafeteria, gymnasium, fitness centre, and student lounges with an average 250+ student enrollment. Specific programming may be expected to adjust as required to provide an identified skilled workforce pipeline into the Huntsman Ocean Park. Cluster building potential given the established presence of marine science capacity in St. Andrews within the adjacent campus of the federal St. Andrews Biological Station (Fisheries and Oceans Canada). St. Andrews provides an idyllic location to operate any business given its position near the mouth of the Bay of Fundy with its magnificent tides. Nearby assets in the Province of New Brunswick are also present to support your business in a global marketplace while operating within the Huntsman Ocean Park, including: Potential to establish manufacturing capacity within the Champlain Industrial Park in the Port of Bayside, NB and St. Stephen Industrial Park adjacent to the USA land border crossing. Easy international access after completing a scenic drive from your choice of four major airports – 1.5 hours from Saint John NB, 2 hrs from Fredericton NB, 2.5 hours from Moncton NB, or 2.5 hours from Bangor ME, USA with international land crossing. Four-lane highway for the majority of the drive from Saint John, NB. Proximity to the Port of Saint John with its international shipping capacity from a significant port complex that occupies 120 hectares of land along 3,900 m of waterfront within Saint John Harbour. Plans to further expand the present rail capacity in Saint John, NB to include a 32-acre modern logistics park to transfer containers between rail cars and tractor-trailers and future terminal to receive and ship goods with the United States and Central Canada. Presence of Cyber Park (Fredericton, NB) and a new innovation district focused on Advanced Health and Energy under development in Saint John, NB that may be leveraged by companies located within the Huntsman Ocean Park. Cluster building potential with RPC as the New Brunswick provincial research organization and provincial Department of Agriculture, Aquaculture and Fisheries. Potential access to Huntsman University Member research capacity, including University of New Brunswick (Saint John and Fredericton campuses), Mount Allison University, and University of Guelph. If you are inspired by the Huntsman mission, wish to discuss your company presence within the Huntsman Ocean Park, or place a study using our professional research services and capacity then please reach out to connect today!

Huntsman Ocean Park …research parks are physical environments that can generate, attract and retain science and technology companies and talent in alignment with sponsoring research institutions that include, universities, as well as public, private and federal research laboratories. Research parks enable the flow of ideas between innovation generators such as, universities, federal labs, and non-profit R&D institutions and companies located in both the research park and the surrounding region. Association of University Research Parks website (August 15, 2021) Research parks drive innovation and economic development – through employment, corporate and individual tax revenue, and spin-off benefits – for its community, region and nation. Research parks provide critical physical space for start-up companies to scale while attracting established corporations to their synergistic benefits focused on a specific sector of the economy. Research parks also cultivate the local S.T.E.A.M. talent pipeline by inspiring high school graduates and serving as a magnet for immigration to a 21st century economy. There has been considerable focus on the ocean economy in Canada over recent years and for good reason. Canada has the longest coastline in the world by far (> 200,000 km) and the fourth largest total ocean area within its jurisdiction. Our collective ocean economy contributes approximately CDN$36 billion annually to Canada’s gross domestic product while employing nearly 350,000 individuals. Fully 75% of this economic activity is located in Atlantic Canada. Even with all of this economic output we still have tremendous opportunity to become global leaders in the ocean economy with staggering local growth potential. The Huntsman Marine Science Centre is stepping up to offer our ocean-focused assets… …welcome to the Huntsman Ocean Park to develop this incredible opportunity in the ocean economy! The Huntsman will leverage its global reputation to support innovation development and validation broadly in precision aquaculture, smart fishing, and ocean digitization by engaging companies to co-locate within the Huntsman Ocean Park through biotechnology, robotics, artificial intelligence, and advanced manufacturing. We are ideally positioned to develop such a cohesive Ocean Park given our: long history to serve as a Contract Research Organization for an esteemed list of local Small to Medium Enterprises (SMEs) to Multinational Corporations (MNCs) as these innovators seek out solutions to meet our greatest challenges and opportunities in the ocean economy; considerable prime land holdings ready for commercial development with community support to do so; research vessel to provide a safe platform for ocean access; and, 20-year ocean institutional lease to facilitate technology development through field deployment and testing Each of these aspects may be explored further by clicking the puzzle pieces within the graphic below. Wine & Dessert Party Learn More Wine & Dessert Party Learn More Learn More Learn More

Huntsman Research Breeding & Genetics Taxonomy Toxicology Animal Health Ocean Assets Visiting Scientist Program More Breeding & Genetics Huntsman is uniquely situated as the only institution in Atlantic Canada, and likely across Canada, that has the experience and capacity to design and manage comprehensive commercially relevant aquatic animal broodstock programs. Our holistic broodstock development approach provides a significant opportunity for the Huntsman to have a measureable impact on local and international aquaculture operations. Contact Amber Garber Dr. Amber Garber provides scientific direction of Huntsman Breeding & Genetics. Amber has been involved in aquaculture and wild fisheries research since 1998 having completed her MSc (University of Southern Mississippi) working on red snapper stock enhancement in the Gulf of Mexico and PhD (North Carolina State University) to advance the hybrid striped bass aquaculture sector. Her research at the Huntsman has focused on Atlantic salmon, Arctic charr, rainbow trout, lumpfish as cleanerfish, Atlantic cod, and sturgeon species. Amber was honoured in 2016 as a recipient of the New Brunswick R3 Innovator Award for her scientific leadership in the Atlantic salmon selective breeding program at the Huntsman alongside industry partner Northern Harvest Sea Farms (today Mowi Canada East). Our Breeding & Genetics effort is supported by an incredible team of technical staff who have decades of experience, often from a commercial production setting, to maintain, handle and assess aquatic individuals as required to collect robust datasets to advance broodstock programs. Huntsman technical staff frequently travel to farm sites elsewhere to complete extensive sampling events that are often associated with spawning, individual fish PIT tagging, sexing by ultrasound, and comprehensive harvest evaluations. If you are inspired to support Huntsman Breeding & Genetics research, advancement of food production, or graduate student research at Huntsman then please donate or choose other ways to support the Huntsman mission today! RESEARCH HIGHLIGHT Our partnership with Huntsman adds a great deal of knowledge and talent to Mowi’s already impressive global research and development capacity. Working in partnership, Mowi and Huntsman are doing genetic selection work to produce the most robust fish for future generations. INSITE – Mowi Canada East Newsletter 9th Edition – October 7, 2020 Broodstock Program Design Broodstock Program Services Controlled Challenges Production Capacity Equipment Example Projects Collaboration with Avademia Selected recent Publications Broodstock Program Design Aquaculture production lags land-based food production in applying methods for deliberate genetic selection of individuals and families displaying favorable performance with commercially relevant production and fitness traits. Our team has tremendous experience to assess your breeding goals and capacity such that a suitable broodstock program is tailor made to meet your specific needs and budget. We have applied our proven SCORE approach across numerous species (e.g., rainbow trout, Arctic charr, sturgeon spp. and shellfish spp.) over the past several years when designing specific broodstock programs, including: State of Knowledge review related to existing broodstock programs and genomics A literature review detailing research related to your species and heritabilities, genetic and phenotypic correlations, breeding programs and published genomic markers. Consultation with industry partners (wants & needs) Arguably the most important step involves open discussions with key individuals within your organization to determine desired outcomes from a broodstock program, including breeding goals, ranking specific traits, and building rapport between the breeding team and Sponsor for open communication. Operational site visit Another critical step involves site visits to better understand the present operations and ensure only viable broodstock program options are recommended based on the facilities, staff capabilities and overall capacity available. Recommendations to implement a broodstock program A description of possible broodstock program strategies will be detailed and options to measure performance of individuals across the range of traits of interest. Execute for project success The execution phase will touch on possible funding models and funding sources that may be approached to move the broodstock program forward to the next stage toward implementation and realizing gains from genetic selection. Broodstock Program Services Effective broodstock programs must be able to reliably collect, manage and analyze data that is directly focused on the specific traits of interest. We must also efficiently keep track of numerous other traits that do not entirely seem pertinent at the time but may be negatively correlated with our genetic selection efforts. Of course, you must have a robust data set coupled with tissue collected from numerous assessed/challenged individuals, families and year classes to effectively take your next steps towards genomic selection. Our team is able to provide a full service in support of genetic selection broodstock programs, including travel to your facilities to complete numerous activities and assessments. Broodstock Handling & Spawning Review Our team has extensive experience appropriately handling broodstock individuals to optimize welfare and increase egg and milt production and quality. We have assessed numerous animal holding facilities and operations with the intent to provide recommendations that have increased fecundity and maximized fertilization rates. Assessments & Spawning Our presence during major broodstock assessment events ensures your staff are able to remain focused on day-to-day farm level operations while specialists support your program needs at your facilities to complete specific year class assessments (e.g., weight/length, deformity assessments, ultrasounding to sex fish), PIT tagging operations, tissue sample collection (e.g., fin clips) and assist with spawning activities (e.g., to create the breeding nucleus and multiplier groups while your staff focus on production crosses). Cryopreservation and Storage of Milt Huntsman has served as the east coast base for Cryogenetics Services Inc. for many years. Milt cryopreserved throughout Atlantic Canada is transferred to the Huntsman where a biosecure facility with controlled access is dedicated for storage of dewars. Cryogenetics have trained Huntsman staff in scheduled maintenance to ensure quality of cryopreserved milt is upheld. Benchmarking Families and Stocks We have completed numerous projects to compare families or stocks across a range of biological, production or fitness traits of interest. These benchmarking efforts must be properly planned and implemented to ensure results are not influenced by other factors. Our experience includes early life history performance (e.g., hatch rates, growth, survival), deformity and growth at PIT tagging size, and controlled challenges involving increasing water temperature, seawater survival, pathogen exposures, etc. Benchmarking also provides an opportunity to involve commercial land-based and sea cage operations of a commercial comparison group that would later be evaluated in the respective company processing plants at harvest to determine differential performance, such as flesh colour, fillet yield etc. Harvest Evaluations As expected, producers tend to focus on production traits that result in shorter time to market (i.e., faster growth) and higher quality product (e.g., fillet colour). Huntsman has established numerous commercial comparison groups that are raised within commercial hatchery and grow-out facilities then evaluated for a host of production and fitness traits. The specific set-up for these evaluations are production specific but typically include aspects of growth, maturation, yield and quality (i.e., colour, texture, composition). Nearly all of the production and fitness traits measured by Huntsman during set harvest evaluations have sufficient heritability to allow improvement through genetic selection. Data Management Software Huntsman developed a proprietary data management software application (referred to as Ceres) that allows advanced relational data entry, collation and management specific to aquatic research activities. Ceres also offers advanced features to manage data, control access by staff responsibilities, and allow remote data uploading capacity. Establishing Breeding Matrix and Cross Design Collected data is statistically analyzed to estimate heritabilities, genetic and phenotypic correlations, and breeding values then used to target the specific family crosses to make during spawning that maximizes genetic make-up of the stock to prevent inbreeding while maximizing genetic gains across the identified traits of interest in a weighted manner of importance. Controlled Challenges Numerous traits will require careful design and execution of phenotypic challenges that ensure the collected data is indeed measuring the actual trait of interest without interference from other factors that may be affecting the challenge outcome. Huntsman has a solid track record of designing controlled challenges that are implemented within our own facilities on the Huntsman campus. Implementing Challenges Performing effective challenges begins with creating a representative random challenge group from the spawning year class to be challenged. Huntsman experience includes directing creation of a random challenge group, acquiring permits to transfer these individuals to the Huntsman campus, and maintaining this stock within appropriate growing conditions until such life stage that the controlled challenge will occur. Specific Challenges Huntsman has impressive infrastructure to supply freshwater and seawater with back-up systems and alarms to offer controlled challenges based on developed protocols that ensure repeatability of results year-over-year to support aquatic broodstock programs. Commonly requested challenges to date include: Climate Change Cultured aquatic species that use the open environment for some aspects of its life cycle will have to cope with increasing temperature conditions. Huntsman has developed protocols that allow collection of data from numerous trials relevant to acute (i.e., CTMax) and chronic (i.e., ITMax) freshwater and seawater temperature increases. Both of these challenges have yielded a high heritability in Atlantic salmon to allow improvement through genetic selection. Seawater Tolerance Smoltification in Atlantic salmon represents a critical life stage that allows transition from freshwater to full salinity conditions; however, this specific process does not technically occur in all salmonids, including rainbow (steelhead) trout. Huntsman has developed protocols to complete a seawater tolerance challenge applicable to collect robust data on fish and families coupled with blood chemistry measurements to allow improvement of stocks through genetic selection. Sea Lice Infestations of sea lice are one of the greatest issues of concern for Atlantic salmon producers globally. Huntsman has developed a reliable and repeatable sea lice infestation model that has been used to complete dozens of challenges to support broodstock programs and novel treatment development options. Sea lice challenges in Atlantic salmon to date have yielded a heritability to allow improvement through genetic selection. Saprolegnia This fungal infection is ubiquitous across virtually all freshwater species production globally. Huntsman has developed a challenge model that produces sufficient spores to reliably infect large volumes of water and numbers of fish and families. Saprolegnia challenges in Atlantic salmon to date have yielded a heritability to allow improvement through genetic selection. Production Capacity Huntsman has extensive facilities to host several commercial scale family-based broodstock programs, with subsequent breeding nucleus and/or production crossing, concurrently. This same production capacity allows us to receive and raise family representation as a random challenge group to complete challenges that are designed to assess a specific trait of interest with the collected data feeding back into the broodstock selection process. Three separate dedicated hatchery facilities with independent recirculating aquaculture systems, temperature control, freshwater and natural seawater availability, and various tank types and sizes. Production capacity available within five Quonset buildings located on campus that together provides more than 2,800 m (more than 30,000 ft ) of controlled access space with freshwater and natural seawater availability. Reliable water flow as either dechlorinated freshwater or natural full salinity seawater with at minimum 50 µm mechanical filtration and UV treatment. Live feed production capacity to support marine species life stages, including 7.5 trillion cells of up to five microalgae species, up to 1 billion rotifers per day, and 100 million Artemia per day production. Support facilities on campus with dedicated biosecure and controlled access for dry food storage, mortality processing, and tissue sample archive. 2 2 Equipment Huntsman has a host of specialized equipment that have been acquired to collect reliable data and measure traits of interest for a specific broodstock program. Portable ultrasound allows sexing of individual fish after gonad sexual differentiation occurs. In most cases, surplus males are culled to provide more tank volume to raise selected females given that egg production is typically the limiting gamete in reproduction. Milt photometer determines sperm cell number within a collected volume of milt. Chemical extender is often used with milt to maintain its viability over 14 days post-collection and an activator may be used when appropriate to allow fertilization of more eggs based on a comfortable ratio of sperm:egg cells. Minolta chromameter supports harvest evaluations as Huntsman is able to quantify fillet colour (i.e., lightness, red-green chromaticity and yellow-blue chromatricity; L*a*b*) and sometimes compare these measurements with the subjective measurement using a Salmofan. We have also used the chromameter to assess quality in other species, such as gonad colour from sea urchins. Near infrared spectrometer to provide chemical analysis of any number of materials and compounds, such as fatty acid, omega-3, pigmentation, etc., following creation of calibration curves for the compound of interest. General laboratory capacity including a suite of equipment (predominantly GLP-compliant) to support research projects including: bench top water distiller, 3-point and 5-point microscale balances, range of calibrated pipettes, autoclave, drying oven, fume hoods, range of glassware, stir plates, various centrifuges and series of mixers. Specific equipment that is not typically mainstream to breeding and genetics, but useful for specific applications is also available from other Huntsman research disciplines (e.g., Coulter counter for ploidy determination). Microscopy including numerous stereo (dissecting) and compound microscopes, coupled with inverted and fluorescence microscopy, with still and video imaging and analysis capacity and digital measurement equipment. Example Projects Below is a sample listing of past and ongoing projects to give an overview of how we have deployed our expertise in Breeding & Genetics to support our Study Sponsors. Study Title Benchmarking of Families and Stocks Assessing early life stage performance of Arctic charr families | Sponsor* - Commercial Creating and assessing founder population to support Atlantic cod production | Sponsor* - Commercial Designing Broodstock Programs Rainbow trout performance improvement strategy | Sponsor* - AANS Sturgeon species broodstock development considerations | Sponsor* - Commercial Supporting Broodstock Activities Spawning activity assessment and training to increase Arctic charr egg production | Sponsor* - Commercial Estimating breeding values to create commercial breeding nucleus crosses | | Sponsor* - Mowi Canada East Controlled Challenges and Assessments Harvest evaluations to support genetic selection of Atlantic salmon | Sponsor* - Mowi Canada East Sea lice, Saprolegnia and increasing seawater temperature challenges | Sponsor* - Commercial Production Post-settled sea urchin production for commercial closed cycle grow-out trials | Sponsor* - Quoddy Savour Seafood Grow-out of lumpfish for stocking as cleanerfish in Atlantic salmon net pens | Sponsor* - Commercial * Sponsors listed by name have previously provided such disclosure on their own or co-authored a publication resulting from the study. Collaborations with Academia We conduct research on a wide variety of Breeding & Genetics topics. At times, we have the privilege of collaborating with academic colleagues by serving on student committees, participating in their projects with our expertise, and publishing research results together. Recent examples of our collaborative academic research in Breeding & Genetics include: Thermal physiology of farmed Atlantic salmon within a commercial breeding program (University of New Brunswick). Mitigating the Impact of Climate-Related Challenges on Salmon Aquaculture (Memorial University of Newfoundland, University of Prince Edward Island and University of Waterloo). Selected Recent Publications Huntsman has always had a strong desire to conduct our own innovative research and contribute to the peer-reviewed literature. This tradition is exemplified by the more than 500 peer-reviewed marine science publications supported by our facilities and expertise to advance scientific knowledge. Our research in Breeding & Genetics continues to be published today as observed by this sample list of recent publications: Reid, G., H.J. Gurney-Smith, D.J. Marcogliese, D. Knowler, T. Benfey, A.F. Garber, I. Forster, T. Chopin, K. Brewer-Dalton, R.D. Moccia, M. Flaherty, C.T. Smith and S. De Silva. 2019. Climate change and aquaculture: considering biological response and resources. Aquaculture Environment Interactions 11:569-602. Reid, G., H.J. Gurney-Smith, M. Flaherty, A.F. Garber, I. Forster, K. Brewer-Dalton, D. Knowler, D.J. Marcogliese, T. Chopin, R.D. Moccia, C.T. Smith and S. De Silva. 2019. Climate change and aquaculture: considering adaptation potential. Aquaculture Environment Interactions 11:603-624. Zanuzzo, F.S., J.A. Bailey, A.F. Garber and A.K. Gamperl. 2019. The acute and incremental thermal tolerance of cod (Gadus morhua) families under normoxia and mild hypoxia. Comparative Biochemistry and Physiology Part A 233:30-38. Garber, A.F., F. Amini, S.A. Gezan, B.D. Swift, S.E. Hodkinson, J. Nickerson and C.J. Bridger. 2019 Genetic and phenotypic evaluation of harvest traits from a comprehensive commercial Atlantic salmon, Salmo salar L., broodstock program. Aquaculture 503:242-253. Marcos-Lopez, M., T.B. Waltzek, R.P. Hedrick, D. Baxa, A.F. Garber, R. Liston, E. Johnson, B. Forward, S. Backman and H.W. Ferguson. 2012. Characterization of a novel alloherpesvirus from Atlantic cod (Gadus morhua). Journal of Veterinary Diagnostic Investigation 24: 65-73. Garber, A.F., J.J. Tosh, S.E. Fordham, S. Hubert, G. Simpson, J.E. Symonds, J.A.B. Robinson, S. Bowman and E.A. Trippel. 2010. Survival and performance of communally reared families of Atlantic cod (Gadus morhua). Aquaculture 307:12-19. Tosh, J.J., A.F. Garber, E.A. Trippel and J.A.B. Robinson. 2010. Genetic, maternal, and environmental variance components for weight and length of Atlantic cod (Gadus morhua) at two life stages. Journal of Animal Science 88:3513-3521. Garber, A.F., S.E. Fordham, J.E. Symonds, E.A. Trippel and D.L. Berlinsky. 2009. Hormone-induced ovulation and spermiation in Atlantic cod (Gadus morhua). Aquaculture 296:179-183.

Huntsman Hospitality Business & Workshops Special Events Large Group Activities Symposia More Business & Workshops Huntsman Marine provides the perfect location to focus and recharge your team in beautiful St. Andrews by-the-Sea! Professionally curated spaces, coupled with options for authentic maritime experiences, are offered to increase employee engagement, enhance teammate collaboration, create strategic visions, develop corporate leadership, conduct a Board meeting or workshop, or simply build mental wellness. Our facilities and amenities are sure to optimize your limited time with us and further enhance what you already expect from our beautiful seaside resort community. We are flexible to provide a customized retreat that can mix and match from our full list of amenities to meet your team’s size, time commitment and budget. Meeting Spaces The heart for hosting small corporate retreats, workshops or focused group meetings exists within the Links Crest Room and adjacent Sunroom in our Anderson House estate on the Huntsman Marine Upper Campus. Year round gatherings are possible within this winterized space that can seat up to 20 participants with all day catered food services, modern audiovisual with virtual attendance capacity, and high speed Wi-Fi internet access all while overlooking Passamaquoddy Bay, our community and the 9th Hole of the Algonquin Golf Course. Huntsman Marine maintains a relationship with professional facilitators in the St. Andrews area who may be engaged to provide third party facilitation and enhance the outcomes from your retreat. Professional Facilitation Catering Meetings hosted on the Huntsman Marine campuses enjoy food service that offers taste, quality and appeal in partnership with Dominion Hill Country Inn. We want guests to experience the passion we have for good food that is well prepared and attractively presented. We will work with you to ensure your participants are well fed, both body and mind, including choices that provide accommodation for special dietary restrictions. Continental breakfasts, morning and afternoon breaks, lunches and dinner menus are all available for meetings planning to stay with us throughout the day. Cocktail hor d’oeurves and cash bar options are also available should you decide to host your group for an evening reception. Overnight Accommodations Multi-day meetings and workshops are easily convened at Huntsman Marine by booking within our block of 20 modern rooms, each with ensuite bathrooms, in Anderson House and the adjacent Nancy Hall . Huntsman Marine Bus We operate an 11-passenger + wheelchair accessible bus that may also be booked to facilitate activities associated with your meeting. R/V Fundy Spray Huntsman Marine operates the R/V Fundy Spray from the St. Andrews Market Wharf. The vessel is a 15.24 m (50 feet) converted offshore lobster boat certified by Transport Canada to carry 20 passengers and two crew. Day-to-day, the vessel provides a spacious platform for scientific diving and well appointed for biological sampling…but also suitable to take a daytime or sunset cruise as a memorable activity for your meeting. Team Building Activities Don’t forget to ask about pricing for our specially curated and unique group activities to provide inspiration, build team cohesion, and clear your mind! Here are some amazing ideas to get your creativity flowing: Up Bay of Fundy Interpretation – explore the Fundy Discovery Aquarium exhibits with a self guided or guided tour with a Huntsman Marine interpreter (Program Length: 1-3 hours) Up Into the Bay of Fundy – increase your knowledge with this great opportunity to touch and hold a series of tide pool animals, such as sea stars, sea urchins, sea cucumbers, etc. under careful guidance by a Fundy Discovery Aquarium interpreter (Program Length: up to 2 hours) Up Buddy Interactions – include our mascot Buddy the Seal to provide fantastic photo opportunities for a truly memorable meeting (Program Length: appearances in 30-minute blocks possible) Up Informative Talk – learn from a 20-minute informative presentation by a Huntsman Marine Research Scientist on a topic of interest to your group either in Anderson House or the Dunn Theatre Up Weave a Keepsake with Discarded Rope – receive group instruction to make your own woven craft in support of a #DebrisFreeFundy ….. and you can add wine for a Wine n’ Weave experience (Program Length: up to 3 hours) Up Walk on the Seafloor – discover the world renown Bay of Fundy intertidal zone with a Huntsman Marine Naturalist followed by an informative lab session (Program Length: up to 3 hours) Up Cruise the Bay – view St. Andrews by-the-Sea and local landmarks from a unique vantage point on the Bay of Fundy aboard the Huntsman Marine R/V Fundy Spray (Program Length: 2-4 hours) Or you can take the short walk from Anderson House directly to the nearby Algonquin clubhouse to play a round on their world class golf course .

Huntsman Conservation #DebrisFreeFundy Marine Protected Areas Marine Invasive Species Environmental Monitoring Citizen Scientist Bird Banding More Marine Protected Areas Marine Protected Areas Huntsman Marine offers extensive Marine Protected Area planning, baseline, and monitoring experience for use within Atlantic Canada and globally. Our experiences may be leveraged to provide a collaborative approach amongst stakeholders ranging from environmental groups and industry to the benefit of all sectors. Marine Protected Areas (MPAs ) are powerful ocean management tools to protect biodiversity and critical habitats while also providing opportunities for tremendous positive spillover effects to local fisheries (e.g., lobsters, scallops, etc.). In 2023, there were 14 MPAs across Canada to protect over 350,000 km2 or 6% of our total marine and coastal waterways. These MPAs are located in our three oceans with the majority of protected area by far off the northwest coast of Ellesmere Island, Arctic Ocean in the Tuvaijuittuq Marine Protected Area established in August 2019 and contributing 5.55% of our national marine conservation target. Coastal waters adjacent to New Brunswick are presently a minor contributor to meet the Canadian target to protect 30% of our ocean habitats by 2030 with only the Musquash Estuary Marine Protected Area encompassing 7 km2 or < 0.01% of our total marine conservation target. While small this MPA has provided protection since 2006 to the largest ecologically intact estuary in the Bay of Fundy. If you are inspired to support Huntsman Marine efforts towards biodiversity and marine protected area research then please donate or choose other ways to support our mission today! Local As noted, the Musquash Estuary Marine Protected Area is the only MPA located in the Bay of Fundy. Huntsman Marine research teammates have participated in monitoring aspects associated with this MPA: Taxonomy & Biodiversity – Huntsman Marine operates a well-established taxonomic identification laboratory, especially focused on benthic and plankton samples from the northwest Atlantic Ocean. We have used this expertise to process benthic samples collected from the Musquash Estuary Marine Protected Area to provide long-term monitoring datasets. Toxicology – Huntsman Marine toxicology capacity has provided seasonal baseline monitoring assessments with Atlantic silverside (Menidia menidia) captured from the Musquash Estuary Marine Protected Area, including condition, reproductive status, and contaminant exposures as determined by the EROD assay. There is also some interest to protect important aquatic habitats within the Fundy Isles region of southwest New Brunswick in the Bay of Fundy. There are over 25 islands within the region with many of them inhabited (e.g., Grand Manan, Campobello, Deer Island) while others are difficult to visit even by boat. This area provides spawning habitat for various fish species amongst many other ecosystem services. Dr. Claire Goodwin has been completing a multi-year effort, funded by the NB Environmental Trust Fun d and Fisheries & Oceans Canada, to survey a series of locations throughout the Fundy Isles that were originally surveyed more than 40 years ago by Art MacKay. The historical and present datasets will also be archived with the Peskotomuhkati Nation to serve as an important baseline of this biodiversity rich area of the Bay of Fundy. Interna tional The largest Marine Protected Areas in the world are located outside of Canada with Huntsman Marine experience also extending to these locations to participate within various benthic SCUBA surveys to provide information for MPA designation and monitoring. These expeditions have occurred in partnership with the South Atlantic Environmental Research Institute with Dr. Claire Goodwin participating as an invited taxonomic expert. Ascension Island Marine Protected Area – In 2019, the entire 445,000 km2 marine zone surrounding Ascension Island was declared to be a Marine Protected Area where no commercial fishing or seabed mining is permitted. Dr. Claire Goodwin visited this MPA has part of an expedition to study the biodiversity using SCUBA. Her efforts included collection of 58 sponge specimens from 17 locations around Ascension Island at depth of 0.5-30 m. Nine new species of demosponges were described to science from this expedition, adding 50% to the number of known species while adding two new genera and on family to the known Ascension Island sponge fauna . The South Georgia and South Sandwich Islands Marine Protected Area (SGSSI-MPA) – The SGSSI-MPA is one of the largest MPAs in the world with an area of 1.24 million km2 – 17 x larger than New Brunswick! This MPA manages about 4.4% of its area as a no take zone to protect the most biodiverse regions of the seabed with the remaining areas managed through a series of strict seasonal and spatial fishery management measures. Dr. Claire Goodwin recently participated in a SCUBA expedition within the SGSSI-MPA and provided an informative blog series of her time there to enhance our public engagement on marine science, environmental monitoring, SCUBA and MPAs. Regional St. Anns Bank Marine Protected Area is located east of Cape Breton Island, Nova Scotia with an approximate size of 4,365 km2, providing approximately 0.08% of Canada’s marine conservation target ). The Huntsman Marine taxonomic identification laboratory has processed benthic samples collected from this MPA as part of a long-term monitoring dataset. The Eastern Shore Islands: Area of Interest (AOI) includes nearshore waters located along the eastern shore of Nova Scotia with an approximate area of 2,000 km2. Huntsman Marine has participated in a joint multi-year SCUBA survey with Fisheries & Oceans Canada to provide baseline data on habitats and sponge biodiversity within this AOI. In 2023, Dr. Claire Goodwin lead the SCUBA survey effort for an Innu Nation Uinipekᵘ Ocean Expedition , supported by Fisheries & Oceans Canada and lead by the Innu Nation and Students on Ice Foundation, and also including researchers from the Canadian Museum of Nature. This SCUBA survey effort provided some of the first scientif ic benthic habitat and species information along this specific region of the Coast of Labrador for the Innu Nation as they assess opportunities to provide additional protections to these important ocean habitats.

Huntsman Overview Vision, Mission & Value Teammates Careers More Huntsman Overview There is no other institution in Canada like the Huntsman – a private not-for-profit social enterprise with an oceans focused mission that also conducts world-class aquatic contract research services. Our storied past is intertwined with giants in the fields of aquatic and fisheries research. And to locate the Huntsman within beautiful St. Andrews-by-the-Sea exemplifies sage wisdom indeed. The Huntsman idea began in 1967, with Dr. John Anderson leading the charge, and testing the concept through the Visiting Investigators Program at the St. Andrews Biological Station in 1968. Success of this program provided further impetus to proceed with legal incorporation of the Huntsman Marine Laboratory in early 1969 as a completely separate, federally incorporated, non-profit, registered charitable organization. Our initial membership involved a consortium of 20 universities and several government departments that came together to become a “co-operative venture in learning.” In 1987, our name was formally changed to The Huntsman Marine Science Centre to better reflect our growth from simply being a field station to operating as an important regional centre, but our overall mission and vision did not change. As we neared our 30th year in 1999, the financial model of the Huntsman presented significant challenges for our long-term sustainability as the university members could no longer sustain the increasing costs to maintain the operations and no single university was willing to step up to assume our overall administrative and financial responsibility. Earlier in 1997, the Future Directions Committee of the Board of Directors had recommended to operate the Huntsman with two separate divisions – an Education and University Research Division and a Contract Research and Development Division. Indeed, Dr. John Anderson wrote in our 1998-99 Annual Report that: “…the more interesting challenge will be to involve University researchers in the conduct of, and delivery to industry, of aquaculture-oriented R&D. This is surely a win/win/win/win opportunity for universities, industry, government, and Huntsman.” His words were focused on the aquaculture industry but today, at the time of this writing in early 2021 and now past 50 years of age, Huntsman is meeting this challenge as we presently operate with two primary “divisions” that together, along with separate Hospitality and Vessel Departments, drive our revenue generation activities: Education & Outreach – Academic (University), Public Education and the Fundy Discovery Aquarium remain our priority areas of activity within this single consolidated department. New to the mix is a focus on the tourism “edutainment” sector (through Huntsman Marine Experiences™ ) and a strong outreach component as a means to capture the imaginations and expose a greater cross-section of society to the importance and wonders of our oceans. Aquatic Biosciences – The aquaculture sector remains one of our top target sectors for on-site contract research activities through Breeding & Genetics but we have also diversified our expertise to include Aquatic Animal Health and Aquatic Toxicology. This department also presently includes Taxonomy & Biodiversity to round out our extensive aquatic research facilities and expertise within a single operational department. The win/win/win/win opportunity as described by Dr. Anderson decades ago has come to fruition as our research activities typically also involve more than 10 graduate students and Post-doctoral Fellows at any given moment in time from numerous institutions across Canada. Huntsman has been privileged to receive numerous organization level and individual awards over the years, including: 2024 Huntsman Marine awarded the Gulf of Maine Council 2024 Visionary Award for New Brunswick in recognition of our wide-ranging achievements as a leader in ocean conservation for the Bay of Fundy and the greater Gulf of Maine watershed through meaningful research, engaging educational programs, and innovative initiatives such as your #DebrisFreeFundy program. 2023 Huntsman Marine awarded with the 2023 Compass Organizational Excellence Award from the Marine Technology Society . This prestigious award recognizes a company, government agency, program, or non-profit organization for outstanding contributions to the advancement of marine science and technology. 2020 Dr. Ben de Jourdan recognized as the 2020 Star Mentor of the Year within the Institution category by the New Brunswick Innovation Foundation R3 awards. 2016 Dr. Amber Garber honoured by receiving a New Brunswick R3 Innovator Award for her scientific leadership in the Atlantic salmon selective breeding program at the Huntsman alongside industry partner Northern Harvest Sea Farms. 2015 Huntsman Marine awarded with a New Brunswick Innovation Research Chair in Aquatic Biosciences position with a focus on aquatic animal health research. 2009 Atlantic Reference Centre received the Gulf of Maine Visionary Award for a New Brunswick organization from the Gulf of Maine Council on the Marine Environment in recognition of its collections and research services. 2000 Huntsman Marine recognized as Canada’s Top Employer of Youth for New Brunswick by the Conference Board of Canada. 1999 Huntsman Marine presented with the Federal Department of Environment EMAN’s “Patricia Roberts-Pichette Award” for enthusiastic leadership and commitment to advancing ecological monitoring and research in Canada. 1991 Huntsman Marine receives the first ever Gulf of Maine Visionary Award by the Gulf of Maine Council on the Marine Environment in recognition of our past accomplishments and unique ability to bring the marine environment and related issues to the forefront of public attention. 1987 Inka Milewki, Public Education Coordinator, selected winner of the James Centorino Award for Distinguished Performance in Marine Education by the National Marine Educators Association. In presenting the award, Dr. Jim Lanier, a colleague from a marine centre in North Carolina noted “that the Huntsman’s Public Education program is unique not only to the Maritimes but to all of Canada.” Huntsman Overview Vision, Mission & Value Teammates Careers More Dr. Archibald Gowanlock Huntsman – Our Namesake The Huntsman Marine Science Centre is named in honour of Dr. Archibald Gowanlock Huntsman (born at Tintern, ON 1883; died at St Andrews, NB 1973). Dr. Huntsman was a preeminent and pioneering oceanographer and marine scientist. He co-authored more than 200 peer-reviewed publications and books on a wide range of marine biology topics and collaborated extensively with colleagues across Canada and internationally. These accomplishments are even more remarkable given that Dr. Huntsman received his formal education with a focus on medicine from the University of Toronto (Bachelor of Arts 1905 and Bachelor of Medicine 1907), although he never practiced medicine. His life’s work in the marine sciences was later recognized with Doctorate degrees honoris causa from Memorial University of Newfoundland (1967) and University of Toronto (1969). In 1911, Dr. Huntsman took on the role as Curator of the fledgling St. Andrews Station on behalf of the Fisheries Research Board of Canada. He was later appointed to be Director in St. Andrews, and held this position until 1934, while concurrently serving as the Director of the newly established Fisheries Experimental Station in Halifax, NS from 1924 to 1928. Dr. Huntsman continued his association with the Fisheries Research Board after his directorship by serving as the editor of its publications from 1934 to 1949 and as a Consulting Director until retiring in 1953. Dr. Huntsman was honoured often by scientific societies in Canada, United States of America and United Kingdom. He was elected to the Royal Society of Canada in 1916, serve as its President in 1938, and received its Flavelle Medal in 1952 “for an outstanding contribution to biological science during the preceding ten years or for significant additions to a previous outstanding contribution to biological science.” His legacy was further solidified in 1980 when the A.G. Huntsman Award for Excellence in the Marine Sciences was established. The award is now recognized as a major international prize, presented by the Royal Society of Canada, to honour marine scientists of any nationality who have had and continue to have a significant influence on the course of marine scientific thought. Dr. Huntsman was also commemorated on 17 March 2000 when Canada Post issued a stamp in his honour – The Fisherman’s Friend – as part of The Millennium Collection. It is very fitting that our institution was named in his honour. In August 1970, Dr. Huntsman was present to cut the ceremonial ribbon during the First Annual Meeting of the Huntsman Marine Laboratory. Dr. W. Bev Scott (another Huntsman Marine Science Centre luminary) wrote that Huntsman’s “…works were truly pioneering and far in advance of contemporary thinking. His writings and discourses characteristically challenged accepted or established thought. Indeed, this attitude of critical appraisal is not only characteristic but also one of his most valuable contributions to science.” We welcome you to explore our website to learn who we are, what we stand for, and see our mission in action to ensure a sustainable future for our coastal communities and ocean resources. A detailed timeline of Huntsman education and research milestones may be accessed from within those specific pages. If you, too, are inspired to support the Huntsman mission then please donate or choose other ways to support the Huntsman mission today!

Huntsman Marine Experiences Fundy Discovery Aquarium Experiences Hourly and Day Trips Multi-Day Experiences More Huntsman Marine Experiences™ Research consistently shows that people are happier after spending money on experiences rather than material things...we couldn’t agree more! Join professional Huntsman Naturalists as we explore life on the seafloor, in the water column, and in the air above the Bay of Fundy! Engagement. Discovery. Inspirational. Educational. These descriptors are tightly woven within the fabric of who we are and what we strive for in Huntsman Marine Experiences™. Our suite of Huntsman Marine Experiences™ provides options for travellers of all ages, budgets, ocean interests, and time commitments. A common thread through most is a paid admission to the Fundy Discovery Aquarium admission pass so you are able to explore Bay of Fundy animals on your own after your focused experience. Hourly and Day Trips provide additional depth as you learn about specific aspects of our marine environment. Multi-day Huntsman Marine Experiences™ are perfect immersive mini-vacations staged from a single base camp to allow you to mentally unwind and grow. In all cases, we remain focused on a core set of objectives when curating and delivering each Huntsman Marine Experience™ to be: Insightful > We are the leaders in providing Bay of Fundy education programs after teaching more than 75,000 students of all ages since 1969. It just seems natural for us to unpack our grade school programs to offer Huntsman Marine Experiences™ to the inquisitive traveller. Intimate > All of our Huntsman Marine Experiences™ target low enrolment numbers of 4-20 participants. Fewer participants ensures more direct interaction for you with our Naturalists and the greater opportunity for you to learn about our incredible Bay of Fundy. Collaborative > We recognize that we may not always have all the assets internally to deliver a specific experience. In such cases, we partner with like minded individuals and organizations to provide you with the highest quality Huntsman Marine Experience™ possible. Impactful > Huntsman is a private not-for-profit ocean focused social enterprise. Our incentive to provide you with an exceptional Huntsman Marine Experience™ is that we get to deliver more programs in marine education , community outreach and collaborative research after you recommend the Huntsman to your family and friends. We look forward to welcoming you soon and promise that you will leave enriched from your specially curated Huntsman Marine Experience™.

Impact Spotlight Studying Effects on American Lobsters Highlights: American lobster (Homarus americanus) exports from Canada reached $3.26 billion in 2021. Other ocean activities and climate change present potential threats to specific lobster life stages. Each year, Huntsman Marine purchases adult lobsters from the local fishery or acquires special permission to retain ‘berried’ (carrying eggs) female lobsters to produce early life stages for toxicology testing. Our American lobster research primarily focuses on completing controlled toxicology exposures to ensure this important fishery is able to co-exist with other important sectors of the ocean economy, such as oil production/shipping and aquaculture production. OceanSteamCourses Summary: The first graduate student conducting research at The Huntsman Marine Science Centre, Dr. Peter Wells, completed some of the earliest studies on the effects of oil spills on American lobster early life stages in the early 1970’s. Today, we offer a globally recognized and robust aquatic toxicology program – studying the effects on numerous freshwater and seawater organisms and life stages following exposure to various established and emerging contaminants. Our capacity to complete professional toxicology exposures across the full range of American lobster life stages and molting phases is globally recognized. Our efforts help to determine risk following use of drugs/pesticides and, in the case of oil spills, help guide the incident response team to choose measures that mitigate effects on American lobster populations. In a typical year, Huntsman Marine research teammates provide husbandry to care for hundreds of adult lobsters and thousands of larval/juvenile lobsters to support these controlled laboratory exposure studies: Pesticides – These compounds often target insects or crustaceans and may also affect American lobsters following sufficient exposure. Larval early life stages live in the water column and are often used in our controlled laboratory exposures with pesticides (e.g., Evaluating non-lethal and latent effects of anti-sea lice pesticide azamethiphos on larvae and adults of the American lobster (Homarus americanus): https://onlinelibrary.wiley.com/doi/abs/10.1111/are.16109 ). Aquaculture drugs – Medicated aquaculture feed has the potential to reach the seabed where lobsters may be living. Huntsman Marine researchers have methods to study the effects of incidental ingestion on lobster survival, molting success and overall health using post-settled juvenile and adult lobsters (e.g., The effects of emamectin benzoate or ivermectin spiked sediment on juvenile American lobsters (Homarus americanus). https://doi.org/10.1016/j.ecoenv.2018.06.075 ). Spilled crude oil and mitigation responses – The East coast of Canada has robust offshore oil production while the Bay of Fundy is an active waterbody for shipping crude oil and refined products in and out of the Port Saint John. From 2015-2022, Huntsman Marine led two comprehensive research programs to investigate the possible effects of an offshore Newfoundland and Labrador oil spill and alternate response measures to mitigate a ship source oil spill on commercially important species, including lobster (e.g., Newly hatched stage I American lobster (Homarus americanus) survival following exposure to physically and chemically dispersed crude oil: https://doi.org/10.1007/s00244-022-00912-z ). Low sulfur fuel oils – In 2022, Huntsman Marine received the prestigious ITOPF Annual R&D Award to study the toxicological effects of new generation low and ultra- low sulfur fuel oils, including on the planktonic lobster life stage. The project also includes a collaboration with Bigelow Laboratory for Ocean Sciences (East Boothbay, ME) while 49 test samples bunkered in 16 countries were provided by the Australian Maritime Safety Authority (AMSA) ( https://www.itopf.org/news-events/news/itopf-grants-11th-annual-research-and-development-r-d-award-to-polite-project-canada/ ). At times our lobster research capacity, coupled with Huntsman Marine aquatic animal health expertise, is also requested to validate aquatic animal holding technologies, such as the case with a recent innovative shipping container study to live haul aquatic animals to distant markets, including the American lobster ( https://oceansupercluster.ca/bluevita-technology-project/ ). Research described in this Impact Spotlight was funded by private sector Study Sponsors, Natural Resources Canada, Fisheries and Oceans Canada, the New Brunswick Innovation Foundation, ITOPF, and Canada’s Ocean Supercluster. Reach out to The Huntsman Marine Science Centre if you have any questions: Toxicology research at Huntsman Marine Contact Dr. Benjamin de Jourdan Aquatic health research at Huntsman Marine Contact Dr. Ehab Misk General inquiries related to Huntsman Marine research capacity Contact Us Explore more Huntsman Marine Impact Spotlights here !

Press Room Oil spill compound effects on culture growth of a marine microalgae species St. Andrews, New Brunswick, Canada – December 3, 2024 Phytoplankton play a critical role in marine ecosystems. They are not only the foundation of marine food webs across the globe, but are also responsible for the production nearly 80% of the global oxygen supply. Microalgae communities also have a high probability of exposure to localized marine oil spills due to their global distribution in the ocean and presence near the surface of the water column. Understanding the effects of oil spills within complex marine ecosystems is quite difficult, as each oil spill is unique and the chemical composition of oil can change in response to different weather conditions. We must therefore focus on building oil spill models that layer validated controlled laboratory data with selected classes of chemical compounds and across a range of species and modulating conditions to better predict ecosystem effects and inform appropriate response strategies. The purpose of this study was to provide data for oil spill models by determining the effects of temperature and UV exposure on toxicity of several different polycyclic aromatic compounds (PACs) found in oil on the marine flagellate Dunaliella tertiolecta (DT). Dr. Danielle Philibert is a Huntsman Marine Associate Research Scientist and lead author on this research publication in the peer-reviewed International Oil Spill Conference Proceedings following its recent presentation at a conference in New Orleans, Louisiana. “Our study builds on past research in the literature that explored PAC toxicity on our same phytoplankton species, but we added the influence of important environmental modifiers, such as temperature and UV light exposure, to mimic exposures in different environmental conditions,” offered Philibert. All experiments were conducted on DT cultured at the Huntsman Marine laboratory, and in their exponential growth phase, to ensure the culture was in good health prior to performing the test. Six individual PACs were chosen for the study to represent a diverse spectrum of chemicals found in oil. Exposures were conducted using a novel passive dosing method to ensure a constant exposure concentration was maintained over the entire trial period. “Traditional exposure methods struggle to maintain a constant PAC exposure concentration over time. Using a partitioned-controlled delivery system with silicone O-rings, our lab is able to complete benchtop scale toxicity exposures over an extended period for up to several days while not having to renew test solution,” explained Philibert. A spectrofluorometer at Huntsman Marine was used to confirm the actual exposure concentrations used for each trial. Temperature tests were completed at 15, 20 and 30 °C while photomodification studies examining the effect of UV light compared test solutions exposed to either 0 or 18 hours of simulated natural sunlight in the lab. “The importance of sunlight to modify toxicity was underappreciated before the Deepwater Horizon oil spill in the Gulf of Mexico. Establishing an artificial solar irradiation chamber in the lab has since opened many research opportunities for our toxicology program,” added Philibert. The scientific endpoint for all trials in this study was microalgae culture growth over the course of the 72 hour test period for each replicate, concentration and PAC. Only two of the PACs tested (fluorenone and 1-methylnaphthalene) caused greater than 50% growth inhibition – an important toxicology statistic referred to as the EC50 – at all three test temperatures while only 1-methylnaphthalene produced results that indicated sensitivity to warmer water temperature. These results mirror prior published studies from the Huntsman Marine lab conducted on lobster larvae. Those studies also found 1-methylnaphthalene toxicity to increase at warmer temperatures, while fluorenone remained unaffected. Exposing the test solutions to UV light had no impact on PAC toxicity with this specific microalgae species. Oil spill models are dependent on having high quality single PAC toxicity data across a range of species and life stages for calibration and validation purposes. This study contributes to these data needs with the microalgae species DT while also expanding our understanding of the impact of environmental modifiers like temperature and UV light on PAC toxicity. “Our results demonstrated that increases in temperature can impact acute toxicity of some PACs to the microalgae species DT but there was relatively minor impact from exposure to UV light. More research is needed to determine if the effects we observed with DT can be replicated with other marine microalgae species,” concludes Philibert. Having these insights are important to initiate further research on the effects of PAC exposure to additional microalgae species given the increasing variability in surface water temperatures due to climate change. The research publication was co-authored by Dr. Benjamin de Jourdan, also a Huntsman Marine Research Scientist. The study was funded through a contribution agreement with Fisheries & Oceans Canada with matching funds provided by industry partners and The Huntsman Marine Science Centre. Research Publication: Philibert, D . and B. de Jourdan . 2024. Generating data for oil spill models: Applications of passive dosing of polycyclic aromatic compounds with marine microalgae. International Oil Spill Conference Proceedings 2024 (1): 238s1. https://doi.org/10.7901/2169-3358-2024.1.238 Image 1: Test vials with culture solutions of the microalgae species and individual oil spill compounds partitioned into silicone O-rings. Image 2: Growth curves of the microalgae species following exposure to two individual oil spill compounds at 15 (blue), 20 (green) and 30 (red) °C clearly showing greater temperature dependency in the test compound graphed in the right panel. Story by Bud Adams, The Huntsman Marine Science Centre. For additional information or images relating to this article, please email huntsman@huntsmanmarine.ca . Return to our Press Room for more stories!

Huntsman Education Universities Schools & Groups Huntsman Marine Academy Children & Families Student Living More Huntsman Marine Academy™ Huntsman Marine Academy™ offers life long learning connections to Huntsman educational programs – including Young Explorer Days & Weeks, Homeschool Ocean Exploration and Ocean S.T.E.A.M.™ for high school students. Each program provides a well deserved Certificate of Completion after the specific program requirements are completed. We welcome you to explore our life long marine science offerings within the tabs below. And, of course, book now to participate in our programs! If you or your company are inspired to support student educational opportunities at Huntsman then please donate or choose other ways to support the Huntsman mission today! Younger Explorer Days & Weeks Homeschool Ocean Exploration Ocean S.T.E.A.M™ Professional Short Courses Young Explorer Days & Weeks Sharks, whales, seals or sea stars…..no matter what your Young Explorer is passionate about, there’s a marine experience waiting at the Huntsman that is sure to surprise and delight! Young Explorer Days & Weeks include interactions with live animals, possible beach exploration, educational games, experiments, themed activities, crafts, and time to explore the Fundy Discovery Aquarium. Pack your lunch, a drink and a snack and don’t forget to bring your rubber boots, jacket, sunscreen and clothing to get wet and dirty! The program fee includes a Fundy Discovery Aquarium admission for the participant. Completion of five (5) day courses or one (1) week course by a single student within the same year will complete the entire program and recognized with a Certification of Completion issued. Age: 5yrs – 11yrs Schedule: Each day course runs from 9:30 am to 2:30 pm. Registration begins at 9:15 am. Numbers: There is a minimum of 5 and maximum of 15 students/program. Themed Days: Lobster Microscopic Life Sharks Sea Stars Seals Whales Seabirds Fishes Weeks: Young Explorer Week 1 Young Explorer Week 2 Book Now Homeschool Ocean Exploration Huntsman welcomes homeschool students to participate in field explorations and hands-on labs focused on our local ocean habitat. Students will receive a Huntsman Marine Academy™ certificate if they complete five days during the season. Completion of five (5) day courses by a single student within the same year will complete the entire program and recognized with a Certification of Completion issued. Schedule: Each day course runs from 10:00 am to 12:30 pm. Registration begins at 9:45 am. Numbers: There is a minimum of 5 and maximum of 12 students/program. Topics may include: Field Trip – Beach Exploration at Indian Point Explore the diversity of animals that live under the rocks and seaweed when the tide goes out. Wear your rubber boots and prepare for a morning of fun searching for crabs, sea stars, urchins, and more! Meet at the Aquarium and we will travel in our own vehicles to Indian Point. Participants are welcome to come back to the Aquarium after (until 12:30pm). Something’s Fishy Dive into the watery world of Bay of Fundy fishes. We will explore adaptations through experimentation, games, and a dissection. Debris Free Fundy Join us in the lab to investigate the impacts of marine debris on ocean animals. Book Now Ocean S.T.E.A.M.™ Welcome to Ocean S.T.E.A.M.™ – High School Career Exploration and Interest Development by Huntsman Marine Academy™! Recent research has shown that just over 80% of graduating high school students felt prepared to enter college/university while only about 50% of students are ready to join the workforce. Our objective with Ocean S.T.E.A.M.™ is to immerse students ages 15-18 years old into week-long courses that focus on S.T.E.A.M. topics in Science, Technology, Engineering, Arts and Mathematics that are relevant to careers within the ocean economy . Benefits from Ocean S.T.E.A.M.™ course participation: Course Descriptions and Registration Small Class Sizes – Each course will have no more than 12 students thereby providing exceptional opportunity to interact with your instructor and make lifelong friends with other teens who share your common interests. Immersive Learning – Enjoy a jam-packed week, living and learning on the Huntsman campus, completing hands-on activities within your chosen Ocean S.T.E.A.M.™ specific course. Explore Career Options – The ocean economy is diverse and a career opportunity may be discovered through your Ocean S.T.E.A.M.™ course activities. Personal Development – Expand on a personal area of interest with instruction from a qualified professional within the specific course subject matter. The Huntsman Marine Science Centre has educated more than 75,000 students of all ages since our beginning in 1969 with a primary focus on aspects related to marine biology and specifically the unique Bay of Fundy region. In 2020, we launched Huntsman Marine Academy™ as a prestigious brand to create lifelong learning connections with the Huntsman, including this immersive Ocean S.T.E.A.M.™ series of short courses designed for high school students and delivered by professionals in relevant fields of the ocean economy. Professional Short Courses Professional Short Courses (tab content on HMA main page similar to Ocean STEAM) Huntsman Marine has exceptional meeting & classroom spaces, overnight accommodations and catering capacity to host Professional Short Courses across a broad range of S.T.E.A.M. topics of interest that benefit graduate students starting out and young & established professionals looking to upgrade in your field. We are not just focused on traditional marine biology topics but include many aspects to enhance your professional life! Course Descriptions and Registration The Huntsman Marine Science Centre has educated more than 80,000 students of all ages since our beginning in 1969. In 2020, we launched Huntsman Marine Academy™ to provide lifelong learning connections between Huntsman Marine and members of all ages within our community. Have a Professional Short Course idea? Building out a robust series of Professional Short Courses requires access to a deep talent pool of knowledgeable instructors with engaging course content in specific areas that demand new knowledge transfer and skills upgrading. If you are a professional, college instructor or university professor with a specific Professional Short Course idea and wish to assess its appropriateness for inclusion within Huntsman Marine Academy™ then please reach out by sending a brief course description to huntsman@huntsmanmarine.ca for an initial assessment.

Huntsman Hospitality Business & Workshops Special Events Large Group Activities Symposia More Symposia Host your conference symposium in a truly memorable venue within the modern soft seat Dunn Theatre in the Fundy Discovery Aquarium while providing lunch and nutrition breaks within the Aquarium Galleries. The Fundy Discovery Aquarium and outside grounds also provide a special ambiance for an evening reception or banquet ceremony in the event that your main conference venue is elsewhere! The Fundy Discovery Aquarium offers the only modern lecture theatre in all of St. Andrews by-the-Sea to host a conference symposium with: free self-parking comfortable soft seat theatre folding tablets on each seat for note-taking professional audiovisual equipment complimentary Wi-Fi for attendees breakfast, nutrition break & lunch catering provided by Dominion Hill Country Inn outdoor space special group experiences to provide a truly unforgettable meeting exclusive access to the underwater life of the Bay of Fundy during your meeting! St. Andrews has served as a resort community for generations and Huntsman Marine has been an integral part of this destination for over 55 years. Visiting St. Andrews today is as easy as completing a scenic drive from your choice of four major airports from: Saint John, NB airport – 1 hour, 20 minutes Fredericton, NB airport – 1 hour, 40 minutes Moncton, NB airport – 2 hours, 40 minutes Bangor, Maine (USA) airport – about 3 hours with international land crossing Upon arrival, it will be clear that your conference symposium will be hosted in a memorable venue within our Fundy Discovery Aquarium and the most unique surroundings on the shores of the Bay of Fundy. The Aquarium Galleries will also provide a special ambiance for nutrition breaks and lunch, provide break out session spaces throughout the facility, or offer an amazing backdrop for an evening reception with our served or self-served cocktail hor d’oeurve for up to 200 attendees or a seated banquet ceremony for 96 guests. St. Andrews is home to many local accommodations throughout our vibrant tourism focused community, including the Algonquin Resort – a 233-room Marriott Autograph Collection hotel. We are also excited to host your conference symposium participants, even if your meeting convenes at another location within St. Andrews, for an evening reception or banquet ceremony as a special event within the Fundy Discovery Aquarium to add a memorable experience for your guests. Don’t forget to include a large group activity for your registrants or as part of your spouse activity programme. Don’t forget to ask about our specially curated and unique group experiences to provide a truly unforgettable meeting for your participants! Here are some amazing ideas to get your creativity flowing: Up Bay of Fundy Interpretation – explore the Fundy Discovery Aquarium exhibits with our interpreters present to answer their questions (Program Length: 1-3 hours and may be timed with your breaks or lunch) Up Into the Bay of Fundy – increase your knowledge with this great opportunity to touch and hold a series of tide pool animals, such as sea stars, sea urchins, sea cucumbers, etc. under careful guidance by a Fundy Discovery Aquarium interpreter (Program Length: up to 2 hours and may be timed with your breaks or lunch) Up Buddy Interactions – include our mascot Buddy the Seal to provide fantastic photo opportunities for a truly memorable meeting (Program Length: appearances in 30-minute blocks possible and may be timed with your breaks or lunch)

Huntsman Research Breeding & Genetics Taxonomy Toxicology Animal Health Ocean Assets Visiting Scientist Program More Aquatic Toxicology The first graduate student at the Huntsman in 1970 studied the effects of oil spills on the American lobster. Today, we combine our extensive experience to hold and close aquatic species life cycles with world class toxicology expertise and facilities to offer exposure studies using published standard methods and custom study designs for a broad range of contaminants and sectors. Contact Benjamin de Jourdan Dr. Benjamin de Jourdan joined the Huntsman team in 2017 to lead our Aquatic Toxicology efforts in support of global Study Sponsors from across sectors of the ocean economy. Ben has tremendous experience across a multitude of species, stressors of interest, exposure systems and statistical methods that is applied within Huntsman research contracts for local, national and international Study Sponsors. Ben was honoured as the recipient of the 2020 Star Mentor of the Year within the Institution category by the New Brunswick Innovation Foundation for his exemplary job of training the next generation of researchers in New Brunswick universities, colleges and research institutions. Huntsman successes in Aquatic Toxicology would not be possible without a deep pool of support technicians to implement the research activities requested of us by our Study Sponsors. Huntsman technical staff have direct expertise to conduct toxicology research, including set-up, implementation and assessments to complete required trials, coupled with years of cumulative experience to acquire and maintain numerous aquatic species and life stages that are cultured to support our studies. If you are inspired to support Huntsman Aquatic Toxicology research, sustainable use of our aquatic resources, or graduate student research at Huntsman then please donate or choose other ways to support the Huntsman mission today! RESEARCH HIGHLIGHT Our government is committed to investing in research to better protect our aquatic ecosystems. In St. Andrews New Brunswick this funding will allow The Huntsman Marine Science Centre to assess the impact of exposure time, temperature and photo- oxidation on the toxicity of individual polycyclic aromatic compounds in several Atlantic marine species. This research will benefit Canadians from coast to coast to coast. The Honourable Dominic Leblanc, President of the Queen’s Privy Council for Canada and Minister of Intergovernmental Affairs Aquatic Toxicology Exposures Contaminants Studied Aquatic Species and Life Stages Endpoints Facilities Equipment Example Projects Collaboration with Academia Selected Recent Publications Aquatic Toxicology Exposures All Huntsman environmental toxicology studies are conducted to meet the principles of Good Laboratory Practices with regards to project management, documentation, quality assurance, and archiving samples and study records. Our expertise and facilities provides significant capacity for the Huntsman to offer toxicology support to Study Sponsors using published standard methods or custom study designs for a broad range of sectors that may affect the aquatic ecosystem. Standard Methods Our facilities are well equipped to complete aquatic standard tests as outlined by Environment Canada, US Environmental Protection Agency, Organization for Economic Cooperation and Development, and any other regulatory agency globally. These studies typically prescribe general or universal conditions and procedures for testing a variety of stressors for their effects on specific species and life stages over a range of concentrations or doses. Industry and government alike require these studies to develop safety data for consumer and commercial products, active pharmaceutical ingredients, and to enforce environmental regulations. Custom Exposure Studies Our ability to develop and implement custom exposure studies, which focus on non-standard but local species that often have cultural and fishery significance, provides further advantage to our Study Sponsors. Huntsman capacity ensures these species and target life stages are available in abundance, amenable to culture conditions, and easy to use within exposure trials. Examples of frequent customized studies include: Exploring the effects of contaminants on early life stages of commercially important species to our region, such as Atlantic cod, American lobster, Atlantic salmon, Atlantic herring and snow crab. Both static and flow-through exposure systems are often used within Huntsman toxicology studies. Delivering study contaminants using novel passive dosing methods are also achieved at the Huntsman on a regular basis. Oral gavage and voluntary feeding methods have been developed to study the effects of in-feed aquaculture medications on non-target species located in the vicinity of fish farm operations, such as juvenile and adult American lobsters. Identifying generational effects following exposure of individuals in collaboration with Huntsman Breeding and Genetics expertise to establish a breeding matrix that, when combined with family-based toxicity data, may detect fisheries recruitment concerns through differential performance within and between families. Research capacity in Aquatic Animal Health and field-based environmental monitoring rounds out our scientific capacity at Huntsman that may be involved to support toxicology studies as required. Contaminants Studied A wide range of contaminants have been studied by our Huntsman team over the past several years. These studies require a series of laboratory resources to support the media preparation, exposure challenge, end point observation, and sample preservation phases for success. Huntsman is well positioned to support studies that are interested in a multitude of chemicals and organisms. Whole Petroleum Products Our team has extensive experience completing hundreds of exposures each year using whole oil products, including offshore Newfoundland crudes, diluted bitumen from Alberta, conventional heavy crudes, and ultra-low-sulfur marine diesel (S ≤0.1 % m/m). Oil Spill Response Measures Incident Command Systems have numerous possible strategies available to address oil spills to limit impact on the receiving environment. Huntsman programs have explored the toxicological effect of these various response measures, including the use of chemical dispersants, herders, in situ burning, and natural attenuation. Single PACs Understanding the impact of single polycyclic aromatic compounds on target species and life stages allows toxicologists to model the effects from whole oil products. The Huntsman team has refined passive dosing methods to study the effects of single and mixtures of PACs and various modulators to advance global oil spill modeling efforts. Drugs and Pesticides Regulatory data collection is required when new drugs and pesticides are developed and registered for use in aquaculture operations. Huntsman has developed a series of exposure methods and end points that allows us to collect these essential data associated with the effects on non-target species, such as the American lobster and benthic community species. Industrial Compounds We all use various compounds in our everyday activities. These compounds require study to determine their environmental effects on species that might be present within receiving waterways. Huntsman is able to complete such product stewardship studies to meet safety data sheet and regulatory submission needs. Effluent Testing Effluent from industrial operations also require continuous monitoring that we are able to complete, particularly as this relates to aquatic non-standard species and life stages. Standard methods are supplemented with specific experimental designs that complement investigation of cause studies. Environmental Contaminants Contaminants are sometimes able to bioaccumulate and their presence within the environment may cause issues for species and communities of aquatic animals even if the point source cannot be identified. Huntsman has capacity to complete certain assays using collected tissue (e.g., EROD) or use collected samples as media to perform controlled exposures in the laboratory setting (e.g., use collected water or sediment for exposures). Aquatic Species and Life Stages Aquatic toxicology studies explore the effects of stressors on species and life stages of interest. Having access to healthy individuals of the target aquatic species and life stage is imperative for environmental toxicology studies. Huntsman has extensive infrastructure that was designed to provide optimal growing and spawning conditions for a suite of freshwater and seawater fish and invertebrates. These same resources offer an exceptional advantage to also support aquatic toxicology research as the required organisms and life stages are produced specifically for each toxicology study. This capacity is essential for the studies that we offer given the need to access non-standard species and life stages that have cultural and commercial importance in the north Atlantic region. American lobster This crustacean species supports an industry throughout all four Canadian Atlantic provinces with a landed value of more than $1B. Annually, Huntsman receives permission to acquire berried (ovigerous) female American lobsters captured during the local spring/summer fishery. These individuals are maintained within our facilities and often assessed to time release and collection of larvae to support exposure trials. Adult lobsters (mixed sex) are also acquired seasonally to support various studies, particularly associated with novel anti-sea lice compounds that require study with the lobster as a commercially important non-target species. Atlantic cod Huntsman maintains wild captured broodstock Atlantic cod that are fed a natural diet throughout the year to ensure conditioning for annual maturation and collection of gametes. Exposure trials have focused on vulnerable early life stages, including gametes, at fertilization, during hatching, and as yolk-sac fry. Sea urchins A commercial fishery exists throughout the Atlantic provinces and Quebec, primarily using diving practices, for the green sea urchin (marketed as “roe” or uni in the Japanese marketplace). We maintain a spawning population of green sea urchins within our facilities so we are able to perform toxicology screening trials year round primarily using the sea urchin fertilization tests with various effluents. This is an important aspect of the Huntsman toxicology program given the seasonal nature to access specific target life stages with our other commercially important species. Atlantic herring The herring fishery has strong cultural and commercial connections with the Bay of Fundy. Seasonal availability of mature Atlantic herring allows the Huntsman to work with the local fishing industry for dockside collections. Fish returned to the lab are assessed for viable gametes to complete exposures on the “sticky” eggs after these are painted onto slides or larvae following successful hatching. Atlantic salmon and rainbow trout Huntsman has extensive experience raising salmonids, including hatchery capacity at a pre-commercial scale. Various freshwater early life stages are seasonally available for Atlantic salmon and rainbow trout to allow specific toxicology studies. Our largest culture tank volumes also provide an opportunity to hold fish that are upwards of 5 kg to support adult exposure trials in seawater, brackish or freshwater conditions. Snow crab Female snow crab carry fertilized eggs for an extended period of time in a manner similar to the American lobster. The Huntsman team has successfully returned ovigerous females to the lab and maintained these individuals until the very delicate larvae are hatched and collected to complete exposures within scintillation vials. A difficult but possible process to study the effects of various contaminants on vulnerable life stages of this commercially important species in the Gulf of St. Lawrence and offshore Newfoundland & Labrador. Oysters and blue mussels Shellfish species have larval life stages followed by a sedentary adult phase thereby presenting a different set of vulnerabilities throughout the entire life cycle for these important cultured species throughout Atlantic Canada. Huntsman toxicology trials have been developed to expose the various life stages with specific stressors based on its presence in the water column or benthic environment. Sea lice The salmon louse, Lepeophtheirus salmonis, are parasitic crustaceans within the order Copepoda. They infect Atlantic salmon in seawater and can cause direct mortalities, reductions in growth and increase the susceptibility of salmon to secondary infections. Huntsman performs assays on sea lice to determine efficacy of existing and new means of chemical control while in culture conditions. Microalgae species Planktonic microalgae serve as the basis of the marine food web. Huntsman maintains several species at scale to serve as food for filter feeders. These same species, along with several others (including a couple of Arctic species), are also maintained at lower volumes in controlled conditions to support toxicology bioassays. Endpoints Huntsman has extensive capacity for collecting and measuring endpoints for toxicity tests, from the cellular to organismal to community levels of interest. Survival and growth Survival and growth are considered some of the most ecologically relevant endpoints in toxicology and are also a staple in regulatory testing requirements. At the Huntsman, we can use environmental controlled chambers and state of the art microscopy to generate survival, growth and development data from a wide range of test species. Respiration Metabolic rate, a sublethal endpoint, plays an important role in the growth, metabolism and activity levels of motile test species. Huntsman has a Loligo respirometer for measuring oxygen consumption and have used microscopy with video recording to measure heart rate, scoring activity levels, and calculating cardiac output. Biomarkers Huntsman has in house capacity to complete molecular assays that elucidate modes of action during toxicity tests and confirm exposures. These include enzymatic assays to measure neurotoxicity and biotransformation (Acetylcholinesterase and Ethoxyresorufin-O-deethylase, respectively) and quantification of relative gene expression (qPCR) for target genes of interest. Modes of Action Response Specific compounds have known modes of action that may be the focus of study. As an example, anti-sea lice drugs and pesticides will have a specific mode of action to affect sea lice survival (e.g., neurotoxicity and molting disruptors) that may be adapted to measure the toxicological effect on non-target species, such as the American lobster. Reproduction Reproductive success plays a critical role in stocking and maintaining species of both economic and cultural importance. Huntsman has experience performing both standard and non-standard reproductive assays using a wide array of test species. Behavioral responses Behavioral responses provide a sub-lethal means to quantify the likelihood of exposure (avoidance vs attraction behavior) or neural impairments that could impact fitness of an organism. Mesocosm studies Huntsman has special capacity to assess the impacts of exposure not only on individual species, but on mini ecosystems housed in mesocosms given our ability to maintain a diverse array of species within our animal holding capacity. Facilities Huntsman has extensive facilities to support numerous concurrent aquatic toxicology studies. Our facilities are designed to provide freshwater or filtered natural seawater, allow temperature control of static bath media across numerous treatments and replicates, and support long-term studies while holding exposed individuals for up to 2 years and beyond for post-exposure monitoring purposes. Two environmental control rooms that allow research teams to complete exposure activities immersed within the same air temperature dictated by the exposure trial details while eliminating the need for use of water baths. Dedicated flow through exposure laboratory that has the capacity to provide continuous exposure to treatment concentrations for extended periods of time using up to 36 test vessels. Solar irradiation chamber that can deliver specific doses of UV light to mimic the impact of the sun on the toxicity and half-life of different petrogenic and pharmaceutical products. UV doses can be validated onsite using both chemical actinometry and full spectrum irradiance meters. Several dedicated live animal holding laboratories with independent recirculating aquaculture systems, temperature control, freshwater and natural seawater availability, and various tank types and sizes, which are typically used for holding broodstock or animals for post-exposure monitoring periods. Dedicated laboratory spaces for exposure media preparation, analytical confirmation of media chemistry, collected sample preparation prior to shipping for further analytical chemistry, glassware cleaning and considerable microscopic capacity. Live feed production capacity to support marine species life stages, including 7.5 trillion cells of up to five microalgae species, up to 1 billion rotifers per day, and 100 million Artemia per day production. Support facilities on campus with dedicated biosecure and controlled access for dry food storage, mortality processing, and tissue sample archive. Equipment Huntsman is well equipped to support professional aquatic toxicology studies that collect reliable and repeatable data that serves our broad base of global Study Sponsors. Laser In-Situ Scattering and Transmissometry (LISST-100X) to quantify the size and distribution of droplets or particles in solution. Fluorometer (Horiba Aqualog, Thermo Scientific Quantech) to determine the concentration of a wide range of organic materials, including aromatics, select pharmaceuticals, and a diverse range of petroleum products following the creation of a calibration curve. Custom flow through test facilities to conduct both acute and chronic toxicity tests in a temperature controlled environment. Solar constant lighting system to replicate both the intensity and emission spectra of sunlight in controlled laboratory conditions for tests involving photosensitive materials. Irradiance meter to measure the intensity of UVA, UVB, and visible light during a toxicity test and critical equipment when conducting tests involving photoactive compounds. Microx reader to non-invasively measure dissolved oxygen concentrations throughout a biological assay and ensure water quality remains above control criteria for the duration of a test. Beckman Multisizer 4e Coulter Counter to quantify unicellular organism density for microalgal cultures as well as provide counts and distribution for sperm, egg, embryos, and blood cells. Loligo respirometer to measure the metabolic rate of a wide range of marine species. The system includes 96 well plates to run tests on small sized organisms, a water bath to maintain consistent temperatures during the assay, and the software to collect and analyze changes in oxygen saturation during the test. qPCR to measure the relative expression of target genes of interest in a wide range of different marine organisms. Epoch 2 microplate spectrophotometer to measure algal culture densities and enzyme activity in a wide range of tissue based bioassays. Microscopy including numerous stereo (dissecting) and compound microscopes, coupled with inverted and fluorescence microscopy, with still and video imaging and analysis capacity and digital measurement equipment. General laboratory capacity including a suite of equipment (predominantly GLP-compliant) to support research projects including bench top water distiller, 3-point and 5-point microscale balances, range of calibrated pipettes, autoclave, drying oven, fume hoods, range of glassware (including baffled flasks), stir plates, various centrifuges and series of mixers. Example Projects Below is a sample listing of past and ongoing projects to give an overview of how we have deployed our expertise in Aquatic Toxicology to support our Study Sponsors. Study Title Whole Petroleum Products Effects of diluted bitumen (dilbit) on early life stages of Atlantic salmon, Salmo salar | Sponsor* - Fisheries and Oceans Canada, National Contaminant Advisory Group Investigation of effects of east coast Canada WAF and CEWAF on early life stages of commercially harvested marine organisms | Sponsor* - Environmental Studies Research Fund Oil Spill Response Measures Essential aquatic toxicology data associated with the deployment of Alternate Response Measures using non-standard species and customized methods and biological endpoints | Sponsor* - Fisheries and Oceans Canada, Multi-Partner Research Initiative International modernization of the CROSERF protocols for oil and dispersed oil ecological research | Sponsor* - Fisheries and Oceans Canada, Multi-Partner Research Initiative Single PACs Toxicology data generation to validate and improve oil spill toxic effects models for fisheries species and at risk life stages in a Canadian context | Sponsor* - Fisheries and Oceans Canada, National Contaminant Advisory Group Single and mixed polycyclic aromatic compound toxicity data generation for the improvement of oil spill models | Sponsor* - Fisheries and Oceans Canada, National Contaminant Advisory Group Pesticides Chronic toxicity of a new pesticide on American lobster (Homarus americanus) | Sponsor* - Commercial Testing the efficacy of a new pesticide against Lepeophtheirus salmonis in vitro | Sponsor* - Commercial Drugs Effects of emamectin benzoate or ivermectin spiked sediment on juvenile American lobsters (Homarus americanus) | Sponsor* - Commercial Lethal and non-lethal effects of a novel anti-sea lice drug at a range of API concentrations on forced fed adult American lobsters (Homarus americanus) | Sponsor* - Commercial Industrial Compounds Long-term and transgenerational effects of exposure to an industrial chemical via partition controlled delivery to oysters | Sponsor* - Commercial Sea urchin (Strongylocentrotus droebachiensis) fertilization in the presence of effluent and process streams from a pulp mill | Sponsor* - Commercial Environmental Contaminants Baseline monitoring of Atlantic silverside (Menidia menidia): Assessment of condition, reproductive status and contaminant exposure as determined by the EROD assay | Sponsor* - Commercial Assessing the avoidance and preference behaviour of Atlantic salmon (Salmo salar) to varying oxygen saturation waters | Sponsor* - GIS Gas Infusion Systems Inc. * Sponsors listed by name have previously provided such disclosure on their own or co-authored a publication resulting from the study. Collaborations with Academia We conduct research on a wide variety of Aquatic Toxicology topics. At times, we have the privilege of collaborating with academic colleagues by serving on student committees, participating in their projects with our expertise, and publishing research results together. Recent examples of our collaborative academic research in Aquatic Toxicology include: Transcriptomics analysis of American lobster (Homarus americanus) larvae after crude oil exposure (McGill University). Toxic effects of dietary uptake of marine oil snow by animals in Newfoundland waters (Memorial University). Assessing the long-term ecological effects of pollutants by characterizing the sub-chronic barriers to recovery in intertidal bivalves exposed to marine diesel oil (University of Guelph). Developing standard toxicity tests with Arctic algae for ecological risk assessment (University of Manitoba). The effects of physically and chemically dispersed crude oil on the metabolic and cardiac performance of commercially important species (University of New Brunswick). Selected Recent Publications Huntsman has always had a strong desire to conduct our own innovative research and contribute to the peer-reviewed literature. This tradition is exemplified by the more than 500 peer-reviewed marine science publications supported by our facilities and expertise to advance scientific knowledge. Our research in Aquatic Toxicology continues to be published today as observed by this sample list of recent publications: Philibert, D.A., T. Parkerton, S. Marteinson, B. de Jourdan. 2021. Assessing the toxicity of individual aromatic compounds and mixtures to American lobster (Homarus americanus) larvae using a passive dosing system. Environmental Toxicology and Chemistry 40: 1379-1388. Boyd, A. C.B. Stewart, D.A. Philibert, Z.T. How, M.G. El-Din, K.B. Tierney and T.A. Blewett. 2021. A burning issue: The effect of organic ultraviolet filter exposure on the behaviour and physiology of Daphnia magna. Science of the Total Environment 750. Philibert, D.A., D.D. Lyons and K.B. Tierney. 2020. Early-life exposure to weathered, unweathered and dispersed oil has persisting effects on ecologically relevant behaviors in sheepshead minnow. Ecotoxicology and Environmental Safety 205: 111289. Kurt-Karakus, P.B., D.C.G. Muir, B. de Jourdan, C. Teixeira, J. Epp Martindale, H. Embers, X. Wang, M. Keir and S. Backus. 2019. Bioaccumulation of selected halogenated organic flame retardants in Lake Ontario. Environmental Toxicology and Chemistry 38: 1198-1210. Daoud, D., A. McCarthy, C. Dubetz and D.E. Barker. 2018. The effects of emamectin benzoate or ivermectin spiked sediment on juvenile American lobsters (Homarus americanus). Ecotoxicology and Environmental Safety 163: 636-645.

Huntsman Conservation #DebrisFreeFundy Marine Protected Areas Marine Invasive Species Environmental Monitoring Citizen Scientist Bird Banding More Environmental Monitoring Huntsman Marine conducts ongoing seawater surveillance, partners with the Province of New Brunswick for air quality and weather monitoring, and provides archival support for the legacy Gulfwatch mussel samples in partnership with Fisheries & Oceans Canada. Gulfwatch Contaminants Monitoring Program Huntsman Marine provides archival support, with funding from Fisheries and Oceans Canada’s (DFO) Marine Environmental Quality Program, for the collected Gulfwatch mussel samples. Gulfwatch was a transboundary chemical contaminants monitoring program organized and administered by the Gulf of Maine Council on the Marine Environment (GOMC). The program ran formally from 1993 – 2012 and involved the collection of blue mussels (Mytilus edulis) at rotating sites in all jurisdictions around the Gulf of Maine (i.e., Nova Scotia, New Brunswick, Maine, New Hampshire and Massachusetts). More details related to the Gulfwatch program may be viewed on the program website here . The existence and preservation of these samples presents an invaluable opportunity to: gain a regional perspective on the distribution and concentrations of toxic contaminants, establish a baseline reference for future monitoring efforts, and improve our understanding of issues that threaten the overall environmental quality of coastal waters within the Gulf of Maine and Bay of Fundy region. Researchers interested in accessing archived samples must submit a research proposal to the Gulfwatch Archive Steering Committee. Proposal submission guidelines must be followed as outlined within the Gulfwatch Management Plan .

Huntsman Conservation #DebrisFreeFundy Marine Protected Areas Marine Invasive Species Environmental Monitoring Citizen Scientist Bird Banding More Marine Invasive Species Marine Invasive Species Huntsman Marine Science Centre recently secured funding from the Department of Fisheries and Oceans Aquatic Invasive Species Prevention Fund to study the distribution and spread of the invasive colonial tunicate ‘sea vomit’ in the Bay of Fundy. What is sea vomit? Sea vomit (Didemnum vexillum) is an invasive colonial sea squirt (a type of marine animal). Native to Japan, it is not naturally found in Canadian waters. When introduced, sea vomit can harm the environment and impact local economies. Sea vomit has spread across the Atlantic and Pacific Oceans, posing a threat to marine ecosystems. Why is sea vomit a problem? Sea vomit grows rapidly and forms large mats. It overgrows native species and can stop their larvae from settling on the seabed. This can include larvae of commercially important species such as scallops. It can be spread through introduction in ballast water, on vessel hulls and marine equipment. It can also spread through fragmentation by mobile gear and weather. The cold water temperatures in the Bay of Fundy have limited its spread but warming seas mean that it is more likely to thrive. Huntsman Marine Project lead Dr Claire Goodwin explains ‘Although scientists recorded sea vomit in the Bay of Fundy in 2013 and 2014, we thought it had died out. But recent SCUBA surveys by our dive team and DFO drop camera surveys in deeper water found extensive populations around Deer Island. This species could severely impact both local marine life and industries. We urgently need more information on its presence in the Bay of Fundy'. How will we study sea vomit? Huntsman will partner with Coastal Action, Passamaquoddy Recognition Group, Fundy North, Fisheries and Oceans Canada and the University of New Brunswick for the three-year study. The team will use a suite of techniques to establish the current distribution and invasion pathways of this harmful species. These include SCUBA surveys, Remotely Operated Vehicle (ROV) surveys, collection of specimens from fishers, monitoring using Environmental DNA (eDNA), and specialised genetic techniques to examine invasion pathways. We will also be looking for other marine invasives species through expert marina surveys and citizen science monitoring. Huntsman Marine Project lead Dr Claire Goodwin explains ‘Although scientists recorded sea vomit in the Bay of Fundy in 2013 and 2014, we thought it had died out. But recent SCUBA surveys by our dive team and DFO drop camera surveys in deeper water found extensive populations around Deer Island. This species could severely impact both local marine life and industries. We urgently need more information on its presence in the Bay of Fundy'. How will we study sea vomit? Huntsman will partner with Coastal Action, Passamaquoddy Recognition Group, Fundy North Fishermen’s Association, Fisheries and Oceans Canada and the University of New Brunswick for the three-year study. The team will use a suite of techniques to establish the current distribution and invasion pathways of this harmful species. These include SCUBA surveys, Remotely Operated Vehicle (ROV) surveys, collection of specimens from fishers, monitoring using Environmental DNA (eDNA), and specialised genetic techniques to examine invasion pathways. We will also be looking for other marine invasives species through expert marina surveys and citizen science monitoring. How can I identify Sea Vomit? Sea vomit is cream or pale yellow. It has channels over its surface. There are often tassel-like growths. It has a rubbery, not spongy texture. Please let us know if you see Sea Vomit - Snap a picture, keep a note of location and date spotted. - Save a sample if possible – samples can be frozen or placed in ethanol. - Email info and image to us at fundyseavomit@gmail.com - Or text us at (506) 607-9148 Marine Invader Monitoring and Information Collaborative (MIMIC) MIMIC is volunteer monitoring effort organized by the Massachusetts Office of Coastal Zone Management (CZM). Since MIMIC began in 2006, hundreds of trained volunteers have searched for marine invasive species at more than 140 sites from Rhode Island to Maine. As part of our project we are setting up new stations in Canada. Or first MIMC monitoring station is in St Andrews. But we are hoping to expand to more sites in New Brunswick and Nova Scotia. Interested in taking part? Please get in touch.