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Press Room Oil spill compound effects on culture growth of a marine microalgae species St. Andrews, New Brunswick, Canada – December 3, 2024 Phytoplankton play a critical role in marine ecosystems. They are not only the foundation of marine food webs across the globe, but are also responsible for the production nearly 80% of the global oxygen supply. Microalgae communities also have a high probability of exposure to localized marine oil spills due to their global distribution in the ocean and presence near the surface of the water column. Understanding the effects of oil spills within complex marine ecosystems is quite difficult, as each oil spill is unique and the chemical composition of oil can change in response to different weather conditions. We must therefore focus on building oil spill models that layer validated controlled laboratory data with selected classes of chemical compounds and across a range of species and modulating conditions to better predict ecosystem effects and inform appropriate response strategies. The purpose of this study was to provide data for oil spill models by determining the effects of temperature and UV exposure on toxicity of several different polycyclic aromatic compounds (PACs) found in oil on the marine flagellate Dunaliella tertiolecta (DT). Dr. Danielle Philibert is a Huntsman Marine Associate Research Scientist and lead author on this research publication in the peer-reviewed International Oil Spill Conference Proceedings following its recent presentation at a conference in New Orleans, Louisiana. “Our study builds on past research in the literature that explored PAC toxicity on our same phytoplankton species, but we added the influence of important environmental modifiers, such as temperature and UV light exposure, to mimic exposures in different environmental conditions,” offered Philibert. All experiments were conducted on DT cultured at the Huntsman Marine laboratory, and in their exponential growth phase, to ensure the culture was in good health prior to performing the test. Six individual PACs were chosen for the study to represent a diverse spectrum of chemicals found in oil. Exposures were conducted using a novel passive dosing method to ensure a constant exposure concentration was maintained over the entire trial period. “Traditional exposure methods struggle to maintain a constant PAC exposure concentration over time. Using a partitioned-controlled delivery system with silicone O-rings, our lab is able to complete benchtop scale toxicity exposures over an extended period for up to several days while not having to renew test solution,” explained Philibert. A spectrofluorometer at Huntsman Marine was used to confirm the actual exposure concentrations used for each trial. Temperature tests were completed at 15, 20 and 30 °C while photomodification studies examining the effect of UV light compared test solutions exposed to either 0 or 18 hours of simulated natural sunlight in the lab. “The importance of sunlight to modify toxicity was underappreciated before the Deepwater Horizon oil spill in the Gulf of Mexico. Establishing an artificial solar irradiation chamber in the lab has since opened many research opportunities for our toxicology program,” added Philibert. The scientific endpoint for all trials in this study was microalgae culture growth over the course of the 72 hour test period for each replicate, concentration and PAC. Only two of the PACs tested (fluorenone and 1-methylnaphthalene) caused greater than 50% growth inhibition – an important toxicology statistic referred to as the EC50 – at all three test temperatures while only 1-methylnaphthalene produced results that indicated sensitivity to warmer water temperature. These results mirror prior published studies from the Huntsman Marine lab conducted on lobster larvae. Those studies also found 1-methylnaphthalene toxicity to increase at warmer temperatures, while fluorenone remained unaffected. Exposing the test solutions to UV light had no impact on PAC toxicity with this specific microalgae species. Oil spill models are dependent on having high quality single PAC toxicity data across a range of species and life stages for calibration and validation purposes. This study contributes to these data needs with the microalgae species DT while also expanding our understanding of the impact of environmental modifiers like temperature and UV light on PAC toxicity. “Our results demonstrated that increases in temperature can impact acute toxicity of some PACs to the microalgae species DT but there was relatively minor impact from exposure to UV light. More research is needed to determine if the effects we observed with DT can be replicated with other marine microalgae species,” concludes Philibert. Having these insights are important to initiate further research on the effects of PAC exposure to additional microalgae species given the increasing variability in surface water temperatures due to climate change. The research publication was co-authored by Dr. Benjamin de Jourdan, also a Huntsman Marine Research Scientist. The study was funded through a contribution agreement with Fisheries & Oceans Canada with matching funds provided by industry partners and The Huntsman Marine Science Centre. Research Publication: Philibert, D . and B. de Jourdan . 2024. Generating data for oil spill models: Applications of passive dosing of polycyclic aromatic compounds with marine microalgae. International Oil Spill Conference Proceedings 2024 (1): 238s1. https://doi.org/10.7901/2169-3358-2024.1.238 Image 1: Test vials with culture solutions of the microalgae species and individual oil spill compounds partitioned into silicone O-rings. Image 2: Growth curves of the microalgae species following exposure to two individual oil spill compounds at 15 (blue), 20 (green) and 30 (red) °C clearly showing greater temperature dependency in the test compound graphed in the right panel. Story by Bud Adams, The Huntsman Marine Science Centre. For additional information or images relating to this article, please email huntsman@huntsmanmarine.ca . Return to our Press Room for more stories!

Atlantic Salmon breeding to Improve Commercial Performance Impact Spotlight Highlights: Atlantic salmon (Salmo salar) aquaculture is a significant food producer and economic contributor in Atlantic Canada – providing 323 million meals and 8,000 jobs each year. Huntsman Marine offers extensive breeding program capacity that assesses the natural variability for traits of interest in a specific population to allow selection and performance improvements with no genetic modification required. We initiated a long-term effort in 2010 that continues today, initially with Northern Harvest Sea Farms and now with Mowi Canada East, to improve performance of commercially important traits within a local Atlantic salmon broodstock population. Downloadable Poster Summary: Our first Huntsman-led research project was associated with establishing the Atlantic salmon farming sector locally back in 1974. In 1983, Huntsman Marine further led a Salmon Genetics Research Program that assessed six New Brunswick river strains for performance in aquaculture production. The Saint John River stock was deemed to be the best and this North American-origin genetic strain still forms the basis today in local fish farming operations. We continue to conduct breeding and genetic selection research to increase productivity, tolerance to a changing culture environment, pathogen resistance and product quality in aquatic farm operations, including Atlantic salmon, Atlantic cod, Arctic charr, rainbow trout, and lumpfish amongst other species. Atlantic salmon is the most significant local farmed fish species and our more recent effort to improve commercial performance began in 2010. In October 2020, Mowi Canada East announced a further 10-year contract with Huntsman Marine to provide local support for this Atlantic salmon population ( https://www.fishfarmingexpert.com/breeding-programme-genetic-selection-huntsman-science-centre/mowi-hires-huntsman-to-track-down-best-broodfish/1282295 ). Our effort within this broodstock program involves: Controlled challenges allowing the Huntsman Marine team to assess performance for very specific traits while controlling other variables that might otherwise affect the observed results. As an example, we have completed yearly assessments since the 2010 year class of this same Atlantic salmon population for resistance to sea lice infestation using controlled challenges to improve performance. Similarly, resistance to the Saprolegnia water fungus was explored after Huntsman Marine developed an appropriate controlled challenge (Resistance to Saprolegnia parasitica infection: A heritable trait in Atlantic salmon: https://doi.org/10.1111/jfd.13664 ). Climate change is expected to affect performance of farmed aquatic animals as the environmental temperature increases and Huntsman Marine developed a suitable controlled challenge to measure tolerance performance and heritability of this trait in Atlantic salmon (in collaboration with UNB Fredericton; Acute critical thermal maximum does not predict chronic incremental thermal maximum in Atlantic salmon (Salmo salar): ( https://doi.org/10.1016/j.cbpa.2022.111143 ). Our subsequent research has estimated high heritability ( https://doi.org/10.1016/j.aqrep.2024.102249 ) and explored the genomic architecture ( https://doi.org/10.1016/j.aquaculture.2023.740020 ) for increasing temperature tolerance in Atlantic salmon. Huntsman Marine has developed standard methods and we are well equipped to visit processing plants globally to complete harvest evaluations and record thousands of data points that measure performance across a series of relevant traits, such as growth, fillet colour, and overall performance (Genetic and phenotypic evaluation of harvest traits from a comprehensive commercial Atlantic salmon, Salmo salar L., broodstock program: https://doi.org/10.1016/j.aquaculture.2019.01.001 ). These assessments have lead to genome wide association studies to identify genomic regions in Atlantic salmon that affects fillet color ( https://doi.org/10.3389/fgene.2024.1402927 ). In 2022, the Government of Canada through Genome Canada and Genome Atlantic announced funding for a collaborative project between Mowi Canada East and Huntsman Marine to map the genomic markers for traits of interest in North American origin Atlantic salmon. ( https://genomeatlantic.ca/new-research-approved-for-atlantic-salmon-aquaculture-and-forestry/ ). Aspects of research described in this Huntsman Marine Science Centre Impact Spotlight were funded by private sector Study Sponsors, the Atlantic Canada Opportunities Agency, the New Brunswick Innovation Foundation, and the Government of Canada through Genome Canada and Genome Atlantic. Reach out to The Huntsman Marine Science Centre if you have any questions: Broodstock research at Huntsman Marine Contact Dr. Amber Garber Aquatic health research at Huntsman Marine Contact Dr. Ehab Misk General inquiries related to Huntsman Marine research capacity Contact Us Explore more Huntsman Marine Impact Spotlights here !

Huntsman Overview Vision, Mission & Value Teammates Careers More In Memory Of We are pleased to play a special role as you choose to honour the life and legacy of a friend, colleague or family member who has passed. Your tribute in memoriam will make a difference towards ocean stewardship, marine education and aquatic research at the Huntsman. Individuals making a donation in honour of a loved one may indicate that you are doing so when completing your donation online. It is also possible to request that a card is sent to the family to let them know that a donation has been made in memoriam. Donate Today Research Donate Today Education & Outreach Donate Today Unrestricted Families may coordinate donations to the Huntsman, in lieu of flowers, in memory of a loved one following their passing. Submitting a brief biography of your loved one is encouraged to post on our website to facilitate in memoriam donations. Huntsman will maintain the specific in memoriam donation page for at least three months. Individuals establishing the in memoriam will be given the opportunity to choose whether the associated donations are transferred for Huntsman Unrestricted, Education & Outreach, or Research Use. If this is not specified then the collected donations will be transferred to the Huntsman Unrestricted Use by default. All donations collected in memoriam that are equal to and greater than $50 will receive a charitable tax credit from The Huntsman Marine Science Centre. Huntsman is grateful to all of our donors whose philanthropic support over decades have built critical infrastructure, enhanced education and research opportunities, and left a legacy of ocean stewardship for the next generation. In Memory of Pat and Noel Tibbo Pat and Noel Tibbo were long-time residents of St. Andrews, and loved the town where they created and raised their family. Noel Tibbo was an internationally renowned fisheries scientist who dedicated his career to fisheries research and management. He and Pat valued the wonder and abundance of our oceans. The family has established this opportunity to donate in memoriam in recognition of their dedication to St. Andrews and their outstanding service to their community and the world of marine science. Donations collected in memory of Pat and Noel Tibbo will be used to support the activities of Huntsman Marine Science Centre. Donate Today In Memory of Dr. Michael David Owen We were saddened to hear of the sudden passing of Dr. Michael David Owen on October 20, 2024. Professor Emeritus Owen was a long time supporter of Huntsman Marine having served as a member of the Board of Directors and teaching several courses here over the years. One of his favourite teaching experiences was instructing the Field Course in Experimental Marine Biology, which he taught along the shores of the Bay of Fundy in New Brunswick on behalf of Western University and Huntsman Marine over many years during his academic career and into his retirement. We are honored that his family has chosen donations to The Huntsman Marine Science Centre in lieu of flowers in his memory. Donate Today

Fundy Discovery Aquarium Visitor Information Accessibility Season Pass/Membership Experiences Arts & Culture Gift Shop More Arts & Culture Art has always played an important role in science. Huntsman Marine continues this tradition, using its facilities and programs to provide intergenerational opportunities for artistic and cultural expression, as we fill a special place within the fabric of southwest New Brunswick society. We look forward to increasing our presence within the local visual & performing arts and cultural activities within our community while also connecting more people to the importance and wonder of our ocean! If you are inspired to support Huntsman Marine arts & culture efforts then please donate or choose other ways to support our mission today! Visual Art – Exhibits The Fundy Discovery Aquarium has a beautiful art exhibition space located just outside of the Dunn Theatre. This gallery provides us with an exceptional opportunity to showcase prints that highlight the intersection where science meets art. We signed a MOU with Bow Seat Ocean Awareness Programs (Westwood, MA) in August 2021 to display Bow Seat youth art submissions within the Fundy Discovery Aquarium Art Gallery for at least one month during our open season each year. The exhibit is coupled with a silent auction fundraiser to support our respective ocean education, inspiration and conservation efforts while allowing our guests to take their favorite prints home. Bow Seat was created to uplift diverse youth voices through programming, art exhibitions, publications, and scholarships that advance dialogue and participation in ocean conservation. Each year, Bow Seat invites youth from around the world to participate in its Ocean Awareness Contest and creatively explore human impacts on the health of our oceans through visual and performing arts, writing, film, and multimedia. Each August we celebrate the activities and accomplishments of The Huntsman Marine Science Centre, including an exhibit within the Art Gallery. This exhibit often showcases scientific illustrations that were prepared for the authoritative Atlantic Fishes of Canada book written by Dr. Bev & Millie Scott while employed at Huntsman Marine. We also use this exhibit for story telling as we communicate our past achievements and present education & research activities. If you are an artist and wish to connect with an exhibition idea for consideration then we encourage you to reach out directly at huntsman@huntsmanmarine.ca . Performing Art – Concerts The Dunn Theatre in the Fundy Discovery Aquarium provides our community with an exceptional performing arts venue. The Aquarium is fully licensed and open for all 200+ concert participants to explore before, during and after each performance. Each concert has attracted amazing singer songwriters – from emerging regional treasures to established internationally acclaimed performers, many of whom are major award nominees and winners. During Paddlefest 2021, we hosted our first concert in this series, in partnership with Periwinkle Productions and Paddlefest, and our list of amazing performances continues to grow with each passing year since then as demonstrated by the past and planned concerts below. If you are a performer or agent and wish to connect with a concert idea then we encourage you to reach out directly to Jamie Steel at mrjamessteel@gmail.com . Concert Performances at the Fundy Discovery Aquarium Harry Manx November 2023 Rose Cousins with CARLENE May 2023 The Salty Sessions – Celebrating the Albums of Salty Towers (featuring Owen Steel, The Olympic Symphonium and Hot Toddy) January 2023 Dave Gunning October 2022 Mo Kenney with Maggie Savoie December 2021 Hot Toddy and Isaac & Blewet September 2021 Old Man Luedecke with Mia Kelly November 2023 John Prine Tribute Show (featuring Brent Mason, Kylie Fox, Sandy Mackay and Wade Hallihan) April 2023 Catherine MacLellan December 2022 Old Man Luedecke and Christina Martin May 2022 Matt Anderson October 2021 Valdy October 2023 Irish Mythen March 2023 William Prince November 2022 Andrea Ramolo March 2022 Paddlefest ’21 Songwriters Circle (featuring Jessica Rhaye, Kylie Fox, Maggie Savoie and Julie Aube) May 2021 Art in Education Programs We are firm believers in the importance of art within science and the ocean economy. Our experiential field programs have often scheduled various history and art connections to expand the learning horizons of visiting students. Our Ocean S.T.E.A.M.™ program includes a specific track of art courses partnership with Sunbury Shores Arts & Nature Centre . Ocean S.T.E.A.M.™ was designed to provide individual high school students with an opportunity to attend an immersive course of interest with other like-minded students and explore future career pathways in the ocean sector, including the visual arts. Culture & Tourism The Fundy Discovery Aquarium is one of the top three tourism attractions within the St. Andrews-by-the-Sea resort community, along with Ministers Island and Kingsbrae Garden – collectively welcoming nearly 100,000 guests each year. Together, our three attractions have partnered to offer visitors a discounted Explore St. Andrews Pass, which has been shown to expand the number of overlapping tourists amongst the three attractions while enticing multi-day activities that benefit the entire local tourism sector. Stay Connected After Your Visit! Follow us on social media (FaceBook , LinkedIn , Instagram and Twitter ), become a Member , sign up to receive the Huntsman Marine Newsletter and plan to visit often.

Huntsman Marine Experiences Fundy Discovery Aquarium Experiences Hourly and Day Trips Multi-Day Experiences More Hourly and Day Trips Learn more about Bay of Fundy marine life from a professional Huntsman Naturalist. This collection of 2-6 hour Huntsman Marine Experiences™ provides adventure options for all ages, interests and time commitments that are just perfect to round out your next St. Andrews by-the-Sea vacation! Huntsman Marine Experiences ™ Huntsman Marine Experiences™ Tidal Trek: Beach Crab Grab Duration 2 hours Starting at $35.00 Learn More Book Now Huntsman Marine Experiences™ Walk Between the Tides Duration 2 hours Starting at $35.00 Learn More Book Now Huntsman Marine Experiences™ Citizen Scientist Marine Cruise Duration 3 hours Starting at $70.00 Learn More Book Now

Huntsman Hospitality Business & Workshops Special Events Large Group Activities Symposia More Business & Workshops Huntsman Marine provides the perfect location to focus and recharge your team in beautiful St. Andrews by-the-Sea! Professionally curated spaces, coupled with options for authentic maritime experiences, are offered to increase employee engagement, enhance teammate collaboration, create strategic visions, develop corporate leadership, conduct a Board meeting or workshop, or simply build mental wellness. Our facilities and amenities are sure to optimize your limited time with us and further enhance what you already expect from our beautiful seaside resort community. We are flexible to provide a customized retreat that can mix and match from our full list of amenities to meet your team’s size, time commitment and budget. Meeting Spaces The heart for hosting small corporate retreats, workshops or focused group meetings exists within the Links Crest Room and adjacent Sunroom in our Anderson House estate on the Huntsman Marine Upper Campus. Year round gatherings are possible within this winterized space that can seat up to 20 participants with all day catered food services, modern audiovisual with virtual attendance capacity, and high speed Wi-Fi internet access all while overlooking Passamaquoddy Bay, our community and the 9th Hole of the Algonquin Golf Course. Huntsman Marine maintains a relationship with professional facilitators in the St. Andrews area who may be engaged to provide third party facilitation and enhance the outcomes from your retreat. Professional Facilitation Catering Meetings hosted on the Huntsman Marine campuses enjoy food service that offers taste, quality and appeal in partnership with Dominion Hill Country Inn. We want guests to experience the passion we have for good food that is well prepared and attractively presented. We will work with you to ensure your participants are well fed, both body and mind, including choices that provide accommodation for special dietary restrictions. Continental breakfasts, morning and afternoon breaks, lunches and dinner menus are all available for meetings planning to stay with us throughout the day. Cocktail hor d’oeurves and cash bar options are also available should you decide to host your group for an evening reception. Overnight Accommodations Multi-day meetings and workshops are easily convened at Huntsman Marine by booking within our block of 20 modern rooms, each with ensuite bathrooms, in Anderson House and the adjacent Nancy Hall. Huntsman Marine Bus We operate an 11-passenger + wheelchair accessible bus that may also be booked to facilitate activities associated with your meeting. R/V Fundy Spray Huntsman Marine operates the R/V Fundy Spray from the St. Andrews Market Wharf. The vessel is a 15.24 m (50 feet) converted offshore lobster boat certified by Transport Canada to carry 20 passengers and two crew. Day-to-day, the vessel provides a spacious platform for scientific diving and well appointed for biological sampling…but also suitable to take a daytime or sunset cruise as a memorable activity for your meeting. Team Building Activities Don’t forget to ask about pricing for our specially curated and unique group activities to provide inspiration, build team cohesion, and clear your mind! Here are some amazing ideas to get your creativity flowing: Up Bay of Fundy Interpretation – explore the Fundy Discovery Aquarium exhibits with a self guided or guided tour with a Huntsman Marine interpreter (Program Length: 1-3 hours) Up Into the Bay of Fundy – increase your knowledge with this great opportunity to touch and hold a series of tide pool animals, such as sea stars, sea urchins, sea cucumbers, etc. under careful guidance by a Fundy Discovery Aquarium interpreter (Program Length: up to 2 hours) Up Buddy Interactions – include our mascot Buddy the Seal to provide fantastic photo opportunities for a truly memorable meeting (Program Length: appearances in 30-minute blocks possible) Up Informative Talk – learn from a 20-minute informative presentation by a Huntsman Marine Research Scientist on a topic of interest to your group either in Anderson House or the Dunn Theatre Up Weave a Keepsake with Discarded Rope – receive group instruction to make your own woven craft in support of a #DebrisFreeFundy ….. and you can add wine for a Wine n’ Weave experience (Program Length: up to 3 hours) Up Walk on the Seafloor – discover the world renown Bay of Fundy intertidal zone with a Huntsman Marine Naturalist followed by an informative lab session (Program Length: up to 3 hours) Up Cruise the Bay – view St. Andrews by-the-Sea and local landmarks from a unique vantage point on the Bay of Fundy aboard the Huntsman Marine R/V Fundy Spray (Program Length: 2-4 hours) Or you can take the short walk from Anderson House directly to the nearby Algonquin clubhouse to play a round on their world class golf course .

Huntsman Research Breeding & Genetics Taxonomy Toxicology Animal Health Ocean Assets Visiting Scientist Program More Taxonomy & Biodiversity Huntsman is recognized as a leader in Canadian Atlantic marine fauna taxonomic and biodiversity services since 1977 when our original Larval Fish Laboratory was established. We offer a full service from study design and field sample collection to sample processing, specimen identification and curation, quality control, and statistical data analysis, interpretation, and reporting. Contact Claire Goodwin Dr. Claire Goodwin's EXPEDITIONS Huntsman Taxonomy & Biodiversity research is led by Dr. Claire Goodwin, who has a wealth of experience in biodiversity research, project management, and data analysis. Claire has over 15 years of experience in benthic taxonomy covering the North Atlantic, Arctic, Antarctic, and sub-Antarctic faunas and has published many scientific papers relating to marine biodiversity. She has particular taxonomic expertise in sponges (Porifera), having described over 70 species new to science. She also brings extensive scientific diving and diving project management experience to the Huntsman team. Huntsman taxonomic work is supported by Rebecca Milne (M.Sc.) as our Taxonomy Projects Supervisor. Rebecca has over 10 years of experience in both benthos and plankton sample processing and marine species identification. She is considered to be an expert in many taxonomic groups but specializes in Crustacea. This expertise is complemented by our team of taxonomic technicians, each with several years of experience in their own taxonomic specializations and many hours within on-site environmental monitoring projects. If you are inspired to support Huntsman Taxonomy & Biodiversity research, discovery of new marine species, or graduate student research at then please donate or choose other ways to support the Huntsman mission today! RESEARCH HIGHLIGHT In May 2020, we provided the first description to science of two new sponge species (Demospongiae: Chalinidae and Suberitidae ) isolated from hyperarid mangroves of Qatar along with research colleagues from Qatar University. Taxonomic Services Biodiversity Service Facilities Equipment Atlantic Reference Centre (ARC) Museum Example Projects Collaborations with Academia Selected Recent Publications Taxonomic Services The Huntsman team is nationally and internationally recognized as a leader in identification of a broad range of faunal groups, including most metazoan aquatic animals spanning the taxonomic range from sponges to fishes. These faunal groups include all life stages, from eggs and larvae to juveniles and adults. We can customize established protocols to suit Study Sponsor needs and budgets. Marine and Freshwater Zooplankton and Ichthyoplankton We have particular expertise in identification of Atlantic and Arctic marine species. Our staff participate in the North-east Atlantic Marine Biological Analytical Quality Control Scheme for zooplankton and scored top marks in the last test. Marine Benthos Our specialties are identification of north-west Atlantic and Arctic macrobenthos. The team is skilled in the identification of infaunal macrobenthos from grabs and cores, epifaunal macrobenthos from trawls and other collections, and analysis of video and still imagery. Freshwater Benthos Staff are certified as project managers for the Canadian Aquatic Biomonitoring Network (CABIN) water quality assessment scheme. Biodiversity Services We provide a full service for research projects from study design and field data collection to analysis, assessment, and reporting. Projects follow established or Sponsor-specified protocols with extensive quality assurance and control (QA/QC) procedures associated with collected data. Field Services The Huntsman is an unequivocal leader in superior sample collection in both marine and freshwater environments. We can provide specimen collection services or advice on sampling protocols to ensure collection of high quality samples. Huntsman is a member of the Canadian Association for Underwater Science and follows the CAUS Standard of Practice for Scientific Diving. Our scientific diving team has experience in management of scientific diving projects, including work in remote locations such as the Antarctic. We also have extensive in situidentification experience in littoral and sublittoral benthos. Data Analysis and Reporting We are experienced in quality control and statistical analysis of large datasets. Our high-quality reports are carefully tailored to meet Sponsor specifications and regulatory requirements. Where required, studies are conducted to meet the standards set out within Good Laboratory Practices with regards to project management, documentation, quality assurance, and archiving samples and study records. Biodiversity Advice We are able to provide scientific advice relating to biodiversity and taxonomy to diverse audiences ranging from government to media productions. Our expertise in species status assessments have been put to use in National General Status programs and Species at Risk Act (SARA) species assessments. Training Huntsman offers taxonomic and other training to technical personnel and students. This can include individual in-person training, provision of workshops and seminars, or creation of identification resources for use on your own. Outreach Our staff are skilled in the creation of press, web, and in-person educational resources and experiences related to biodiversity and natural history. We possess an extensive photographic catalogue of north-west Atlantic species that may be used for media and education purposes. Facilities Spacious modern laboratory equipped with stereo (dissecting) and compound microscopes, calibrated balances, microscope imaging, digital measurement equipment, and custom ventilation system. Comprehensive library of taxonomic identification resources, built up over our long operational history and continually updated. Controlled access sample archive room with temperature-monitored refrigerators, freezers, and ultra-low temperature freezer (-86oC). Additional controlled storage for chemically preserved samples. Sample collection, working closely with Huntsman Ocean Operations , from a safe and stable platform anywhere in the Bay of Fundy onboard our R/V Fundy Spray in support of environmental monitoring projects. The Fundy Sprayis a 15.24 m (50 feet) converted offshore lobster boat that is certified by Transport Canada to carry 20 passengers and 2 crew. The vessel is well appointed with winches suitable to support sediment grab sampling, towing plankton nets for collection, bottom trawling and dragging, trap retrieval, and deployment of other research equipment. It also serves as a spacious platform for scientific diving. Equipment General laboratory capacity including a suite of equipment (predominantly GLP-compliant) to support research projects including: bench top water distiller, 3-point and 5-point microscale balances, range of calibrated pipettes, autoclave, drying oven, fume hoods, range of glassware, stir plates, various centrifuges and series of mixers. Microscopy including numerous stereo (dissecting) and compound microscopes, coupled with inverted and fluorescence microscopy, with still and video imaging and analysis capacity and digital measurement equipment. Field sampling equipment for both marine and freshwater environments including a range of benthic grabs (suitable for any study proposal or sediment type), Van Dorn and Niskin water samplers, plankton nets, kick nets, and portable geochemistry monitoring equipment. SCUBA equipment with our own on-campus air compressor and high quality SLR underwater camera system. Atlantic Reference Centre (ARC) museum The Atlantic Reference Centre (ARC) museum is a joint venture that is co-owned and operated by Huntsman and Fisheries and Oceans Canada (DFO) since its inception in 1984. The ARC houses the most extensive curated museum collection of marine fauna from the Canadian Atlantic, including approximately 150,000 professionally preserved, catalogued, and digitized specimen lots ranging from sponges to fishes collected from estuaries and coastal waters to the deep sea. Collection records are made publically available through the Ocean Biodiversity Information System . Tours and loans of specimens may be available by request. In 2009, the Gulf of Maine Council on the Marine Environment awarded the ARC its Gulf of Maine Visionary Award for a New Brunswick organization in recognition of its collections and research services to understand and protect marine life. Huntsman taxonomists use the museum as a comprehensive reference collection to ensure identification accuracy for our taxonomy and biodiversity projects. Study Title Marine Zooplankton and Ichthyoplankton Identification of zooplankton samples from the Newfoundland region as part of long-term monitoring from 1990 to the present. | Sponsor* - Fisheries and Oceans Identification of zooplankton samples from the Gulf of Maine. | Sponsor* - CanadaWoods Hole Oceanographic Institution Marine Benthos Analysis of grab samples collected from around Atlantic Canada as part of the Aquaculture Modelling and Monitoring Programm. | Sponsor* - Fisheries and Oceans Infaunal and epifaunal benthic impact assessment for oil rig disposal with a focus on invasive species. | Sponsor* - Commercial client Freshwater Analysis of freshwater macroinvertebrate samples for CABIN database. | Sponsor* - Non-profit organization Freshwater environmental impact study looking at impact of pharmaceutical product to fulfill Environment Canada requirements. | Sponsor* - Commercial client Biodiversity Assessments Providing General Status Ranks under the Species at Risk Act for marine fish and invertebrate species in the Atlantic Region. | Sponsor* - Fisheries and Oceans Characterisation of the sublittoral habitats of the Brier Island/Digby Neck Ecologically and Biologically Significant Area (SCUBA survey and reporting). | Sponsor* - Fisheries and Oceans Outreach and Education Selected by the Canadian Wildlife Federation to conduct a BioBlitz of Passamaquoddy Bay, representing the Atlantic coast of Canada as part of a national campaign to celebrate Canada’s 150th year. | Sponsor* - Canadian Wildlife Federation Training workshop on shipboard identification of fish and invertebrate species collected on trawl surveys. | Sponsor* - Fisheries and Oceans * Sponsors listed by name have previously provided such disclosure on their own or co-authored a publication resulting from the study. Example Projects Below is a sample listing of past and ongoing projects to give an overview of how we have deployed our expertise in Taxonomy & Biodiversity to support our Study Sponsors. Collaborations with Academia We conduct research on a wide variety of Taxonomy & Biodiversity topics. At times, we have the privilege of collaborating with academic colleagues by serving on student committees, participating in their projects with our expertise, and publishing research results together. Dozens of queries are posed to our taxonomic specialists every year from academia and other sectors located from around the world. Recent examples of our collaborative academic research in Taxonomy & Biodiversity include: ICY-LAB (Isotope Cycling in the Labrador Sea) Co-ordinated by Dr Kate Hendry, University of Bristol. Includes supervision of a PhD student working on sponge ecology and taxonomy. Census of Marine Life’s Natural Geography in Shore Areas (NaGISA) program. An international collaboration resulting in six publications. Research on the effects of microplastics on the plankton communities in Saint John Harbour and community change. Funded by Fisheries and Oceans Canada and involving academic collaborators from the University of New Brunswick and University of Waterloo. Collaboration with the South Atlantic Environmental Research Institute on taxonomy of sponges in the sub-Antarctic regions and SCUBA survey of sublittoral habitats. Selected Recent Publications Huntsman has always had a strong desire to conduct our own innovative research and contribute to the peer-reviewed literature. This tradition is exemplified with the publication of Atlantic Fishes of Canada in 1988 by Dr. Bev Scott, former director of the Huntsman Marine Science Centre, and Milly Scott from their extensive curatorial research completed at the Huntsman – a book still viewed as the preeminent Canadian fish-bible by professionals to this day. Our research in Taxonomy & Biodiversity continues to be published today as observed by this sample list of recent publications: Van Guelpen L., C. Goodwin, R. Milne, G. Pohle and S. Coutenay. 2021. Distribution and structure of coastal ichthyoplankton communities of the Bay of Fundy in southern New Brunswick, Canada. Marine Biodiversity 51(2) . Culwick, T., J. Phillips, C. Goodwin, E.J. Rayfield and K. Hendry. 2020. Sponge Density and Distribution Constrained by Fluid Forcing in the Deep Sea. Frontiers in Marine Science. Giraldes, B.W., C. Goodwin, N.A.A. Al-Fardi, A. Engmann, A. Leitão, A.A. Ahmed, et al. 2020. Two new sponge species (Demospongiae: Chalinidae and Suberitidae) isolated from hyperarid mangroves of Qatar with notes on their potential antibacterial bioactivity. Goodwin, C., J. Berman and K. Hendry. 2019. Demosponges from the sublittoral and shallow-circalittoral (<24 m depth) Antarctic Peninsula with a description of four new species and notes on in situ identification characteristics. Zootaxa 4658: 461–508. Cooper, J.A., C. Goodwin, P. Lawton, T. Brydges, C. Hiltz, S. Armsworthy and Q. McCurdy. 2019. Characterisation of the sublittoral habitats of the Brier Island/Digby Neck Ecologically and Biologically Significant Area, Nova Scotia, Canada. Canadian Technical Report of Fisheries and Aquatic Sciences 3327: xv + 163 p. Goodwin, C., B.E. Picton, C.C. Morrow and P.E.J. Dyrynda. 2017. Chapter 3: Porifera. In Handbook of the Marine Fauna of North-West Europe 2nd edition (P. J. Hayward and J. S Ryland, eds.). Oxford University Press. Pohle, G., K. Iken, K.R. Clarke, T. Trott, B. Konar, J.J. Cruz-Motta, M. Wong, L. Benedetti-Cecchi, A. Mead, P. Miloslavich, N. Mieszkowska, R. Milne, L. Tamburello, A. Knowlton, E. Kimani and Y. Shirayama. 2011. Aspects of Benthic Decapod Diversity and Distribution from Rocky Nearshore Habitat at Geographically Widely Dispersed Sites. PLoS ONE 6: e18606. Milne, R. and C.L. Griffiths. 2013. Additions to and revisions of the amphipod (Crustacea: Amphipoda) fauna of South Africa, with a list of currently known species from the region. African Natural History 9: 61-90. Van Guelpen, L, D.F. Markle and D.J. Duggan. 1982. An evaluation of accuracy, precision, and speed of several zooplankton subsampling techniques. ICES Journal of Marine Science 40(3) 226-236.

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Ocean Test Site In July 2021, Huntsman secured a 20-year Institutional Lease for a nearby 56-acre ocean test site. Having long-term access to an ocean test site provides considerable stability and predictability to companies locating within the Huntsman Ocean Park as: Innovative knowledge-based companies focused on aspects of the ocean economy are able to position their engineers/scientists, software developers, prototype fabricators, and production functions amongst others within an ecosystem of like minded companies co-locating in the Huntsman Ocean Park land development . All of these companies retain a privileged position as Study Sponsors with the Huntsman whereby their innovative products and breakthroughs are confidentially developed, validated, and perfected within existing Huntsman aquatic research facilities . Access to the ocean for field testing of innovative products is provided by the Huntsman Coast Guard certified R/V Fundy Spray with its extensive gear deployment and sampling capacity. Our ocean test site allows companies to confidentially deploy its innovative products with ease to ensure it is market ready with all of the appropriate development, prototyping, testing, and validation within the Huntsman Ocean Park prior to commercialization. The size of our ocean test site ensures we are able to accommodate numerous companies concurrently while also ferrying your staff and prospective customers to the site to view your innovation in action. If you are inspired by the Huntsman mission, wish to discuss your company presence within the Huntsman Ocean Park, or place a study using our professional research services and capacity then please reach out to connect today!

Multi-Day Experiences Huntsman Marine Experiences Fundy Discovery Aquarium Experiences Hourly and Day Trips Multi-Day Experiences More St. Andrews by-the-Sea – A Place like No Other 5 days / 4 nights / 10 meals Date TBA St. Andrews by-the-Sea – A Place like No Other $1150 single occupancy $1080 double occupancy $690 staying off campus (includes full program of activities with lunches, dinners and socials but overnight and breakfast elsewhere then self-driving to shuttle stop locations) SYNOPSIS St. Andrews is rich with fascinating people, architecture and history. From First Nations residents, French explorers and English loyalists, to today’s multicultural mix, St. Andrews is a town like no other. Early shipping, colonial wars, railway tycoons, the rich and famous, tourism, pioneering marine science and with a constantly evolving arts and culture scene, St. Andrews punches well above its weight for its size. The Algonquin Hotel was Canada’s first seaside resort, the town’s “Maxwell Houses” are Canada’s largest collection of heritage homes designed by Edward and William Maxwell, the waterfront is designated a National Historic District and Water Street has been named one of the “Great Streets” in the country. Canadian marine science got its start here and research continues at both the federal St. Andrews Biological Station and The Huntsman Marine Science Centre. Today, our tiny town with a year round population of around 2,000 attracts visitors, new residents, scientists, visual and performing artists, and entrepreneurs from around the world. This Huntsman Marine Experience TM will open your eyes to this one of a kind community. ACTIVITY LEVEL: Fair Most of our activity will be walking in town (pavement) or on lawns and gravel roads. Generally flat with minimal change in elevation but recommended to wear good walking shoes. HIGHLIGHTS - Interact with numerous local celebrity history buffs whose link to the area goes back generations as well as newbies who have come by choice – including a number of well known local authors and curators - Go behind the scenes with local expert historians on guided tours of historic sites – including churches, Maxwell Houses, Ministers Island and hidden treasures in the collections of local museums - Explore local history like never before from the water aboard the Huntsman Marine R/V Fundy Spray Book Now Schedule at a Glance Detailed Itinerary Location Details What to Bring Guides Participant Life Schedule at a Glance This Huntsman Marine – Encounter with Nature™ provides an immersive cultural experience with a full schedule, having one activity flowing into the next, and will include evening sessions. Field trips are a go “rain or shine.” The following schedule at a glance is provided as an inspirational guide, but we may have to juggle activities based on weather conditions for each day. Day 1 Arrival/settle-in. Orientation. Meet your Guides. (group dinner, evening social) Day 2 Passamaquoddy Nation. Early French Explorers. Boat excursion on St. Croix River and Passamaquoddy Bay. (breakfast, lunch, dinner, evening social) Day 3 Town Plat walking tour of architecture, churches, museums, Courthouse and Old Gaol. (breakfast, Niger Reef lunch, dinner on your own) Day 4 Historical tour continues of hotel and Maxwell Houses. Ministers Island. (breakfast, Kingsbrae Garden lunch, soiree dinner, evening social) Day 5 Goodbyes and Departures. (breakfast)

Huntsman Hospitality Business & Workshops Special Events Large Group Activities Symposia More Special Events Impress your guests as you host a sustainable holiday party, retirement gathering, wedding ceremony or wedding party reception, fundraising gala, product launch, or award ceremony with private access to the entire Fundy Discovery Aquarium ! It isn’t everyday that you get to mingle and celebrate amongst the underwater residents of the Bay of Fundy. Our team of professionals are able to transform the Fundy Discovery Aquarium to meet your special event needs. Venues Both the Anderson Gallery and Flemer Gallery are on the first floor of the Fundy Discovery Aquarium and able to comfortably host 150 guests in a reception style event or 72 for a more formal seated dining experience. Seasonally our beautiful grounds, viewing decks and low tide intertidal beach access provide additional fabulous event space for your guests to enjoy. Food & Beverage The Huntsman is committed to work towards a #DebrisFreeFundy and our event catering practices reflect this in our approach to serve menu items in the absence of personal single-use plastics throughout our Huntsman Marine Hospitality operations. Food and beverage stations may be placed throughout our Galleries and grounds (seasonally and weather permitting) to facilitate strolling dinner events. The Fundy Discovery Aquarium and its grounds are licensed to provide options for wine pairings or operate a cash bar during your special group event. (Please note that bar service (alcohol) must conclude at least thirty (30) minutes prior to the scheduled end of your event) Optional 'Edutainment' Activities Interpretation – Huntsman naturalists and interpreters will be available to engage your guests throughout the Fundy Discovery Aquarium Galleries and exhibits, including animal facts and inner workings to operate the Aquarium facility (Program Length: Up to 3 hours). Snorkel Interactions – Our mascot Snorkel will interact with your guests and provide fantastic photo opportunities (Program Length: Two 30-minute appearances possible). Into the Bay of Fundy – Enhance your event with this great opportunity to allow your guests to touch and hold a series of tide pool animals, such as sea stars, sea urchins, sea cucumbers, etc., under careful observation of a Fundy Discovery Aquarium Naturalist/Interpreter (Program Length: Up to 2 hours). Diver Interaction – Guests are provided a unique opportunity to observe a diver within the Bay of Fundy tank and interact with your guests through the viewing glass (Program Length: One 30-minute dive). Informative Talk – Guests enjoy a 20-minute informative presentation in either the Anderson Gallery or Dunn Science Theatre that may be tailored for: History of the Huntsman to Fulfill Our Ocean Mission Towards a #DebrisFreeFundy Biodiversity of the Bay of Fundy We hope you choose to host your next special event at the Fundy Discovery Aquarium in beautiful St. Andrews by-the-Sea! Your special event will support the Huntsman ocean mission to inspire stewardship, advance marine sciences through collaborative research, and deliver inspirational educational experiences. Frequently Asked Questions Is there a discounted rate for different nights of the week or during the off-season? Yes, discounted rates are offered for Fundy Discovery Aquarium rentals and food and beverage minimum for special events that take place during our off-season (October through April) and on Sunday – Tuesday inclusive during May through September. When do you require a final guest count for a special event? The final guest count must be communicated to your Huntsman point of contact seven business days prior to your special event. If you are offering preselected entrée options to your guests then the final breakdown of meals must also be provided at this time. What is the deposit payment schedule? You will make three separate payments before your special event hosted at the Fundy Discovery Aquarium. The initial non-refundable deposit is due upon signing the contract equal to 33% of the contracted total to save your date. The second deposit of 33% will be due three months before the special event to provide your firm commitment. The final 34% payment is due seven business days before the special event and coincides with your final guest count. Does the Fundy Discovery Aquarium host on-site wedding ceremonies? Yes, we are able to host ceremonies both indoors throughout the Fundy Discovery Aquarium or outside on our grounds. The ceremony fee includes event staff for the ceremony, ceremony rehearsal (time and location subject to availability), chairs, speakers, and a microphone. Additional fees apply for ceremonies that begin before 7:30 p.m. during the months of July and August. Is there a food and beverage minimum order? Yes, a food and beverage minimum is required for all private special events hosted at the Fundy Discovery Aquarium and varies based on the type of event and space required. Are there limitations in the way my special event may be decorated? Yes, certain guidelines apply for decorations, flower arrangements and conduct during your special group event to ensure safety and well-being of all Fundy Discovery Aquarium animals and consistent with our mission to protect the ocean environment. We specifically prohibit elastic bands, rubber/silicone wristbands, balloons, glow items such as sticks, bracelets, necklaces, Hawaiian leis, toothpicks, skewers, stirrers, certain plant materials, cans or bottles, marbles, sand, small toys, ribbon, Mylar, confetti, glitter, rice, birdseed, live fish, and dried marine life. Seashells are allowed in vases only. Candle flames must be enclosed by glass (for example, hurricane lamps or votive holders). Please discuss your decorating options with our knowledgeable staff. Does the Fundy Discovery Aquarium have audiovisual capacity to support a special event? Yes, the Anderson Gallery is equipped with a projector/screen and audio to ensure your presentation is a success. Of course, the Dunn Science Theatre may also be used for presentations within the comfort of a modern theatre setting. Are outside caterers permitted to serve food or beverages? No, all food and beverages must be provided by Huntsman Marine Hospitality. Does the Huntsman provide set-up furniture for my special event? Yes, Huntsman offers complimentary house tables, chairs and a set of linens that we will set-up for a specific number of guests attending your special group event. We are happy to also discuss accommodation of specialty linen and upgraded chairs for use during your event. Am I permitted to play amplified music within the Fundy Discovery Aquarium? Yes, but amplified music will be monitored to remain within a reasonable sound level for the safety of our animals. Band restrictions may apply for indoor special group events.

CONTACT US If you're interested in learning more about the Huntsman, our courses, programs, facility, resources, or how you can become involved, please fill out the contact form below and we will be in touch. Submit 1 Lower Campus Road St. Andrews, New Brunswick Canada E5B 2L7 506-529-1200

Huntsman Marine Experiences Fundy Discovery Aquarium Experiences Hourly and Day Trips Multi-Day Experiences More Fundy Discovery Aquarium Behind the Scenes Tour This Huntsman Marine Experience™ will immerse you into our local marine life and coastal heritage while discovering the magnitude of the world’s HIGHEST tides! Overview Location Reviews Overview Duration 1 hour Price Adults: $28.00 +HST (and fees) Senior/Student: $26.00 +HST (and fees) Children (8yrs+): $23.00 +HST (and fees) Includes full-day pass to the Fundy Discovery Aquarium About A knowledgeable interpreter will take you behind the locked doors of the Fundy Discovery Aquarium to our favorite points of interest. You will have the opportunity to feed some animals, learn about our animal care protocols to keep the animals healthy, including the Life Support Systems that filters and recirculates our pumped ashore natural seawater. Some of our Aquarium animals are only found in our behind-the-scenes tanks! You will also learn more about the broad array of Huntsman activities, including research and education. The animals themselves are always changing Behind-the-Scenes based on Aquarium activities, so there is always something unique to see and learn! Book Now

This hands-on course will introduce students to the tools they need to understand mechanical movement by focusing on the foundations of simple machines, physics, and engineering design with direct application to ocean industries. Activities will engage students to engineering fields in a fun and interactive style, allowing them to design and innovate with their newly acquired engineering principles. Course Descriptions and Registration Huntsman Education Universities Schools & Groups Huntsman Marine Academy Children & Families Student Living More Ocean S.T.E.A.M.™ Exploring Engineering Design for Ocean Applications Date TBD This hands-on course will introduce students to the tools they need to understand mechanical movement by focusing on the foundations of simple machines, physics, and engineering design with direct application to ocean industries. Activities will engage students to engineering fields in a fun and interactive style, allowing them to design and innovate with their newly acquired engineering principles. Example Career Connections: Mechanical Engineer, Oceanographer, Renewable/Alternative Energy Technologist, High School Science Teacher Apply Today Schedule at a Glance Narrative Schedule Location Details Course Instructor What to Bring Student Life Schedule at a Glance Course participants will explore the scientific methods and engineering design process, develop appropriate strategies for logical problem solving, and learn about engineering principles and energy. This program is very hands-on and the days are full, with one activity flowing into the next, and will include evening sessions. The course is structured to introduce core concepts followed by activities to reinforce each concept with a focus on ocean applications. The following schedule is provided as an inspirational guide, but we may have to juggle activities based on weather conditions for each day. Day 1 Arrival/settle-in. Orientation. Day 2 Simple vs complex machines. Boat trip to explore oceanography problem solving. Day 3 Alternative energy. Tour of Huntsman Fundy Discovery Aquarium. Day 4 Pressure and pneumatics. Day 5 Scientific design process. Day 6 Presentations. Clean lab. Group photo. Wrap-up. schedule Narrative Location

Huntsman Research Breeding & Genetics Taxonomy Toxicology Animal Health Ocean Assets Visiting Scientist Program More Research Breeding & Genetics Huntsman is uniquely situated as the only institution in Atlantic Canada, and likely across Canada, that has the experience and capacity to design and manage comprehensive commercially relevant aquatic animal broodstock programs. Our holistic broodstock development approach provides a significant opportunity for the Huntsman to have a measureable impact on local and international aquaculture operations. Learn More Taxonomy & Biodiversity Huntsman is recognized as a leader in Canadian Atlantic marine fauna taxonomic and biodiversity services since 1977 when our original Larval Fish Laboratory was established. We offer a full service from study design and field sample collection to sample processing, specimen identification and curation, quality control, and statistical data analysis, interpretation, and reporting. Learn More Toxicology The first graduate student at the Huntsman in 1970 studied the effects of oil spills on the American lobster. Today, we combine our extensive experience to hold and close aquatic species life cycles with world class toxicology expertise and facilities to offer exposure studies using published standard methods and custom study designs for a broad range of contaminants and sectors. Learn More Animal Health Aquatic pathogens and parasites must be addressed to ensure aquatic food production and security. Our ability to maintain an array of finfish and shellfish species and life stages in freshwater and natural seawater allows us to customize study designs – focused on efficacy, target animal safety, residue depletion, and regulatory data collection – that tackle the most pressing aquatic animal health issues of our time. Learn More Ocean Assets Huntsman is the only marine focused non-government education and research institution located on the shores of the Bay of Fundy – including New Brunswick and Nova Scotia. We also provide students and researchers a safe and stable platform to conduct studies and work anywhere in the Bay of Fundy onboard our Coast Guard certified R/V Fundy Spray with its extensive sampling capacity. Learn More Visiting Scientist Program The Huntsman Marine Visiting Scientist Program provides an opportunity for early career and senior scientists to collaborate with our extensive expertise while staying and conducting research on the Huntsman Marine campus in St. Andrews, NB. Learn More Huntsman Research Huntsman is unique in Canada as an ocean focused social enterprise that also serves as a best-in-class aquatic Contract Research Organization. After more than 50 years, Huntsman continues to create a significant impact through world-class aquatic research, as exemplified by the more than 500 peer-reviewed marine science publications supported by our facilities and expertise. Learn More